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MALDI-target integrated platform for affinity-captured protein digestion

Ahmad Tajudin, Asilah and Adler, Belinda and Ekstrom, Simon and Marko-Varga, Gyorgy and Malm, Johan and Lilja, Hans and Laurell, Thomas (2014) MALDI-target integrated platform for affinity-captured protein digestion. Analytica Chimica Acta, 807 . pp. 1-8. ISSN 0003-2670; ESSN: 1873-4324

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To address immunocapture of proteins in large cohorts of clinical samples high throughput sample processing is required. Here a method using the proteomic sample platform, ISET (integrated selective enrichment target) that integrates highly specific immunoaffinity capture of protein biomarker, digestion and sample cleanup with a direct interface to mass spectrometry is presented. The robustness of the on-ISET protein digestion protocol was validated by MALDI MS analysis of model proteins, ranging from 40 fmol to 1 pmol per nanovial. On-ISET digestion and MALDI MS/MS analysis of immunoaffinity captured disease-associated biomarker PSA (prostate specific antigen) from human seminal plasma are presented.

Item Type:Article
Keyword:SPE; MALDI; Proteomics; PSA; Immunoaffinity; Digestion
Faculty or Institute:Faculty of Biotechnology and Biomolecular Sciences
DOI Number:10.1016/j.aca.2013.08.051
ID Code:34429
Deposited By: Nurul Ainie Mokhtar
Deposited On:15 Dec 2015 11:02
Last Modified:15 Dec 2015 11:02

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