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A process for producing lipase

Raja Noor Zaliha Raja Abd. Rahman. (2013) A process for producing lipase.

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Abstract

A process for producing lipase by microorganisms on the industrial scale, wherein Escherichia coli microorganism harbouring lipase, preferably thermostable lipase, is fermented under batch or fed batch conditions, in a Luria Bertani (LB) broth supplemented with citric acid, (NH4)2SO4, Na2HPO4.12H2O, MgCl2, NH4Cl and glucose, using a trace metal solution containing hydrochloric acid, FeSO4.7H2O, ZnSO4.7H20, CaCl2.H20, MnSO4.5H20, CuSO4.5H2O, AlCl.6H2O, and H3BO3, and a feeding nutrient solution containing glucose, yeast extract, MgSO4.H2O, KH2PO4, Citric Acid, (NH4)2SO4, Na2HPO4.12H2O, MgCl2, and NH4Cl, at dissolved oxygen tension of 30-50 %, at temperature of 30-40 °C, while maintaining the pH between 6 and 8, with a glucose content of 15-25 g/L, with isopropylthiogalactoside.

Item Type:Patent
Keyword:Producing lipase; Microorganisms
Faculty or Institute:Faculty of Biotechnology and Biomolecular Sciences
Application Number:PI2013700818
Filing Date:2013-05-20
ID Code:33645
Deposited By: Mohd Hafiz Che Mahasan
Deposited On:17 Mar 2015 11:54
Last Modified:27 Sep 2016 09:19

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