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A Spectrophotometric Method for the Determination of Glucose with Glucose Oxidase [E.C. 1.11.1.7] Using Titanium (IV)-4-(2'-pyridylazo)Resorcinol Reagent

Ampon, Kamaruzaman and Wan Yunus, Wan Md Zin and Salleh, Abu Bakar (1994) A Spectrophotometric Method for the Determination of Glucose with Glucose Oxidase [E.C. 1.11.1.7] Using Titanium (IV)-4-(2'-pyridylazo)Resorcinol Reagent. Pertanika Journal of Science & Technology, 2 (1). pp. 47-53. ISSN 0128-7680

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Abstract

A mixture of Ti(IV) and 4-(2-pyridylazo) resorcinol was found to be useful in the spectrophotometric determination of trace amounts of hydrogen peroxide. The absorbance of the complex formed at 508 nm was proportional to the concentration of hydrogen peroxide added. The reagent was applied to the assay of glucose through coupling with glucose oxidase which produces HP2' This method of glucose determination was rapid, convenient and showed minimal interference from reducible substances.

Item Type:Article
Keyword:spectrophotometric determination, hydrogen peroxide, Ti-PAR reagent, glucose oxidase
Faculty or Institute:Faculty of Environmental Studies
Publisher:Universiti Putra Malaysia Press
ID Code:3107
Deposited By: Nur Izzati Mohd Zaki
Deposited On:24 Nov 2009 09:54
Last Modified:27 May 2013 15:05

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