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Fluorescent quantitation method for differentiating the nativity of green fluorescent protein


Citation

Chew, Few Ne and Tan, Wen Siang and Tey, Beng Ti (2011) Fluorescent quantitation method for differentiating the nativity of green fluorescent protein. Journal of Bioscience and Bioengineering, 111 (2). pp. 246-248. ISSN 1389-1723; ESSN: 1347-4421

Abstract

A gel imaging method was employed to quantitate the GFP that had been subjected to denaturation and degradation treatments. This method is able to differentiate the nativity of GFP by relating the observed changes in the position of fluorescent bands which is unable to be detected using the spectrofluorometric method.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Faculty of Engineering
Institute of Bioscience
DOI Number: https://doi.org/10.1016/j.jbiosc.2010.10.004
Publisher: The Society for Biotechnology, Japan
Keywords: Degradation; Denaturation; Fluorescence quantitation; Gel imaging method; Green fluorescent protein; Nativity; Spectrofluorometer
Depositing User: Nabilah Mustapa
Date Deposited: 07 Dec 2015 02:44
Last Modified: 07 Dec 2015 02:44
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1016/j.jbiosc.2010.10.004
URI: http://psasir.upm.edu.my/id/eprint/23106
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