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Virus-like particles of Macrobrachium rosenbergii nodavirus produced in bacteria


Citation

Zee, Hong Goh and Tan, Soon Guan and Bhassu, Subha and Tan, Wen Siang (2011) Virus-like particles of Macrobrachium rosenbergii nodavirus produced in bacteria. Journal of Virological Methods, 175 (1). pp. 74-79. ISSN 0166-0934

Abstract

Macrobrachium rosenbergii nodavirus (MrNv) infects giant freshwater prawns and causes white tail disease (WTD). The coding region of the capsid protein of MrNv was amplified with RT-PCR and cloned into the pTrcHis2-TOPO vector. The recombinant plasmid was introduced into Escherichia coli and protein expression was induced with IPTG. SDS-PAGE showed that the recombinant protein containing the His-tag and myc epitope has a molecular mass of about 46kDa and it was detected by the anti-His antibody in Western blotting. The protein was purified using immobilized metal affinity chromatography (IMAC) and transmission electron microscopic analysis revealed that the recombinant protein assembled into VLPs.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Faculty of Science
DOI Number: https://doi.org/10.1016/j.jviromet.2011.04.021
Publisher: Elsevier
Keywords: Virus-like particles; Nodavirus; Nodaviridae; Cloning; Purification; Expression
Depositing User: Najwani Amir Sariffudin
Date Deposited: 10 Feb 2015 01:11
Last Modified: 23 Nov 2015 02:58
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1016/j.jviromet.2011.04.021
URI: http://psasir.upm.edu.my/id/eprint/22325
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