Optimized lipase catalyzed synthesis of adipate ester in a solvent-free system
Chaibakhsh, Naz and Abdul Rahman, Mohd Basyaruddin and Abd. Aziz, Suraini and Basri, Mahiran and Salleh, Abu Bakar and Raja Abdul Rahman, Raja Noor Zaliha (2009) Optimized lipase catalyzed synthesis of adipate ester in a solvent-free system. Journal of Industrial Microbiology & Biotechnology, 36 (9). pp. 1149-1155. ISSN 1367-5435; ESSN: 1476-5535
Immobilized Candida antarctica lipase-catalyzed esterification of adipic acid and oleyl alcohol was investigated in a solvent-free system (SFS). Optimum conditions for adipate ester synthesis in a stirred-tank reactor were determined by the response surface methodology (RSM) approach with respect to important reaction parameters including time, temperature, agitation speed, and amount of enzyme. A high conversion yield was achieved using low enzyme amounts of 2.5% w/w at 60C, reaction time of 438 min, and agitation speed of 500 rpm. The good correlation between predicted value (96.0%) and actual value (95.5%) implies that the model derived from RSM allows better understanding of the effect of important reaction parameters on the lipase-catalyzed synthesis of adipate ester in an organic solvent-free system. Higher volumetric productivity compared to a solvent-based system was also offered by SFS. The results demonstrate that the solvent-free system is efficient for enzymatic synthesis of adipate ester.
Repository Staff Only: Edit item detail