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Optimized lipase catalyzed synthesis of adipate ester in a solvent-free system

Chaibakhsh, Naz and Abdul Rahman, Mohd Basyaruddin and Abd. Aziz, Suraini and Basri, Mahiran and Salleh, Abu Bakar and Raja Abdul Rahman, Raja Noor Zaliha (2009) Optimized lipase catalyzed synthesis of adipate ester in a solvent-free system. Journal of Industrial Microbiology & Biotechnology, 36 (9). pp. 1149-1155. ISSN 1367-5435; ESSN: 1476-5535

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Immobilized Candida antarctica lipase-catalyzed esterification of adipic acid and oleyl alcohol was investigated in a solvent-free system (SFS). Optimum conditions for adipate ester synthesis in a stirred-tank reactor were determined by the response surface methodology (RSM) approach with respect to important reaction parameters including time, temperature, agitation speed, and amount of enzyme. A high conversion yield was achieved using low enzyme amounts of 2.5% w/w at 60C, reaction time of 438 min, and agitation speed of 500 rpm. The good correlation between predicted value (96.0%) and actual value (95.5%) implies that the model derived from RSM allows better understanding of the effect of important reaction parameters on the lipase-catalyzed synthesis of adipate ester in an organic solvent-free system. Higher volumetric productivity compared to a solvent-based system was also offered by SFS. The results demonstrate that the solvent-free system is efficient for enzymatic synthesis of adipate ester.

Item Type:Article
Keyword:Lipase; Adipate ester; Solvent-free system; Optimization; Response surface methodology
Faculty or Institute:Faculty of Biotechnology and Biomolecular Sciences
Faculty of Science
Institute of Bioscience
DOI Number:10.1007/s10295-009-0596-x
ID Code:15995
Deposited By: Nida Hidayati Ghazali
Deposited On:16 Jul 2012 12:52
Last Modified:02 Sep 2016 12:39

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