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Isolation of trinucleotide microsatellite markers for Mystus nemurus

Hoh , Boon Peng and Siraj, Siti Shapor and Tan, Soon Guan and Yusoff, Khatijah (2008) Isolation of trinucleotide microsatellite markers for Mystus nemurus. Russian Journal of Genetics, 44 (3). pp. 369-371. ISSN 1022-7954

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Abstract

Twelve single-locus trinucleotide microsatellite markers were developed to characterize the Asian river catfish, Mystus nemurus, an important food fish in Southeast Asia. They were obtained by using a rapid method, namely, the 5′ anchored PCR enrichment protocol. The specific primers were designed to flank the repeat sequences and these were subsequently used to characterize 90 unrelated fish from Malaysia. The number of alleles per locus ranged from 2 (MnVj2-281) to 12 (MnBp8-4-43b) while the levels of heterozygosity ranged from 0.0444 (MnVj2-1-19) to 0.7458 (MnVj2-291).

Item Type:Article
Keyword:Trinucleotide microsatellite
Subject:Catfishes - Research
Subject:Microsatellites (Genetics)
Subject:Genetic markers
Faculty or Institute:Faculty of Biotechnology and Biomolecular Sciences
Publisher:Springer
ID Code:15335
Deposited By: Nida Hidayati Ghazali
Deposited On:16 Aug 2012 09:19
Last Modified:08 Nov 2012 12:14

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