Isolation of trinucleotide microsatellite markers for Mystus nemurus
Hoh , Boon Peng, Siraj, Siti Shapor, Tan, Soon Guan and Yusoff, Khatijah (2008) Isolation of trinucleotide microsatellite markers for Mystus nemurus. Russian Journal of Genetics, 44 (3). pp. 369-371. ISSN 1022-7954
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Twelve single-locus trinucleotide microsatellite markers were developed to characterize the Asian river catfish, Mystus nemurus, an important food fish in Southeast Asia. They were obtained by using a rapid method, namely, the 5′ anchored PCR enrichment protocol. The specific primers were designed to flank the repeat sequences and these were subsequently used to characterize 90 unrelated fish from Malaysia. The number of alleles per locus ranged from 2 (MnVj2-281) to 12 (MnBp8-4-43b) while the levels of heterozygosity ranged from 0.0444 (MnVj2-1-19) to 0.7458 (MnVj2-291).
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