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Improvement of folate biosynthesis by lactic acid bacteria using response surface methodology

Muhamad Nor, Norfarina and Mohamad, Rosfarizan and Foo, Hooi Ling and Abdul Rahim , Raha (2010) Improvement of folate biosynthesis by lactic acid bacteria using response surface methodology. Food Technology and Biotechnology, 48 (2). pp. 243-250. ISSN 1330-9862

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Abstract

Lactic acid bacteria (Lactococcus lactis NZ9000,Lactococcus lactis MG1363, Lactobacillus plantarum I-UL4 and Lactobacillus johnsonii DSM 20553) have been screened for their ability to produce folate intracellularly and/or extracellularly. L. plantarum I-UL4 was shown to be superior producer of folate compared to other strains. Statistically based experimental designs were used to optimize the medium formulation for the growth of L. plantarum I-UL4 and folate biosynthesis. The optimal values of important factors were determined by response surface methodology (RSM). The effects of carbon sources, nitrogen sources and para-aminobenzoic acid (PABA) concentrations on folate biosynthesis were determined prior to RSM study. The biosynthesis of folate by L. plantarum I-UL4 increased from 36.36 to 60.39 mg/L using the optimized medium formulation compared to the selective Man de Rogosa Sharpe (MRS) medium. Conditions for the optimal growth of L. plantarum I-UL4 and folate biosynthesis as suggested by RSM were as follows: lactose 20 g/L, meat extract 16.57 g/L and PABA 10 mM.

Item Type:Article
Keyword:Folate; Lactic acid bacteria; Lactobacillus plantarum I-UL4; Response surface methodology
Subject:Lactic acid bacteria
Subject:Biosynthesis
Subject:Experimental design
Faculty or Institute:Faculty of Biotechnology and Biomolecular Sciences
Publisher:Elsevier B.V.
ID Code:15142
Deposited By: Nida Hidayati Ghazali
Deposited On:16 Aug 2012 11:00
Last Modified:31 Oct 2012 11:45

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