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Improvement of folate biosynthesis by lactic acid bacteria using response surface methodology

Muhamad Nor, Norfarina and Mohamad, Rosfarizan and Foo, Hooi Ling and Abdul Rahim, Raha (2010) Improvement of folate biosynthesis by lactic acid bacteria using response surface methodology. Food Technology and Biotechnology, 48 (2). pp. 243-250. ISSN 1330-9862; ESSN: 1334-2606

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Abstract

Lactic acid bacteria (Lactococcus lactis NZ9000,Lactococcus lactis MG1363, Lactobacillus plantarum I-UL4 and Lactobacillus johnsonii DSM 20553) have been screened for their ability to produce folate intracellularly and/or extracellularly. L. plantarum I-UL4 was shown to be superior producer of folate compared to other strains. Statistically based experimental designs were used to optimize the medium formulation for the growth of L. plantarum I-UL4 and folate biosynthesis. The optimal values of important factors were determined by response surface methodology (RSM). The effects of carbon sources, nitrogen sources and para-aminobenzoic acid (PABA) concentrations on folate biosynthesis were determined prior to RSM study. The biosynthesis of folate by L. plantarum I-UL4 increased from 36.36 to 60.39 mg/L using the optimized medium formulation compared to the selective Man de Rogosa Sharpe (MRS) medium. Conditions for the optimal growth of L. plantarum I-UL4 and folate biosynthesis as suggested by RSM were as follows: lactose 20 g/L, meat extract 16.57 g/L and PABA 10 mM.

Item Type:Article
Keyword:Folate; Lactic acid bacteria; Lactobacillus plantarum I-UL4; Response surface methodology
Faculty or Institute:Faculty of Biotechnology and Biomolecular Sciences
Institute of Bioscience
Publisher:Faculty of Food Technology and Biotechnology, University of Zagreb, Croatia
ID Code:15142
Deposited By: Nida Hidayati Ghazali
Deposited On:16 Aug 2012 11:00
Last Modified:14 Aug 2017 11:35

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