Effects of plant growth regulators on callus induction from Cananga odorata flower petal explants

Nurazah, Z. and Muse, Radzali and Ahmad, Syahida and Mahmood, Maziah (2009) Effects of plant growth regulators on callus induction from Cananga odorata flower petal explants. African Journal of Biotechnology, 8 (12). pp. 2740-2743. ISSN 1684–5315

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Abstract

The Cananga odorata callus was initiated from petals of the C. odorata flowers on MS medium and B5 vitamins containing 30 g/L sugar and 3 g/L agar. The medium was also supplemented with different concentrations of 1-naphtalene acetic acid (NAA), and combinations of NAA with 6-benzylaminpurine(BAP) as the plant growth regulators. It was observed that the C. odorata callus could be induced on media containing the combination of 3 mg/L NAA and 0.5 mg/L BAP. High concentration of NAA gave rise to pale, whitish and friable callus after 1 - 2 weeks of culture. The optimum pH for the cell culture was about 5.7 and incubation at 25 ± 2°C, in totally dark condition.

Item Type:Article
Keyword:Cananga odorata; 1-naphtalene acetic acid; 6-benzylaminopurine; Callus culture
Subject:Cananga odorata
Subject:Growth (Plants)
Subject:Cell culture - Laboratory manuals
Faculty or Institute:Faculty of Biotechnology and Biomolecular Sciences
Publisher:Academic Journals
ID Code:14401
Deposited By: Nida Hidayati Ghazali
Deposited On:16 Aug 2012 07:59
Last Modified:02 Nov 2012 01:12

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