Simple Search:

Effects of plant growth regulators on callus induction from Cananga odorata flower petal explant


Citation

Zain, Nurazah and Muse, Radzali and Ahmad, Syahida and Mahmood, Maziah (2009) Effects of plant growth regulators on callus induction from Cananga odorata flower petal explant. African Journal of Biotechnology, 8 (12). art. no. 00AA34E8081. pp. 2740-2743. ISSN 1684–5315

Abstract / Synopsis

The Cananga odorata callus was initiated from petals of the C. odorata flowers on MS medium and B5 vitamins containing 30 g/L sugar and 3 g/L agar. The medium was also supplemented with different concentrations of 1-naphtalene acetic acid (NAA), and combinations of NAA with 6-benzylaminpurine(BAP) as the plant growth regulators. It was observed that the C. odorata callus could be induced on media containing the combination of 3 mg/L NAA and 0.5 mg/L BAP. High concentration of NAA gave rise to pale, whitish and friable callus after 1 - 2 weeks of culture. The optimum pH for the cell culture was about 5.7 and incubation at 25 ± 2°C, in totally dark condition.


Download File

[img] PDF
14401.pdf
Restricted to Repository staff only

Download (142kB)

Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Publisher: Academic Journals
Keywords: Cananga odorata; 1-naphtalene acetic acid; 6-benzylaminopurine; Callus culture
Depositing User: Nida Hidayati Ghazali
Date Deposited: 16 Aug 2012 15:59
Last Modified: 14 Nov 2017 15:54
URI: http://psasir.upm.edu.my/id/eprint/14401
Statistic Details: View Download Statistic

Actions (login required)

View Item View Item