Nurazah, Z. and Muse, Radzali and Ahmad, Syahida and Mahmood, Maziah (2009) Effects of plant growth regulators on callus induction from Cananga odorata flower petal explants. African Journal of Biotechnology, 8 (12). pp. 2740-2743. ISSN 1684–5315
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The Cananga odorata callus was initiated from petals of the C. odorata flowers on MS medium and B5 vitamins containing 30 g/L sugar and 3 g/L agar. The medium was also supplemented with different concentrations of 1-naphtalene acetic acid (NAA), and combinations of NAA with 6-benzylaminpurine(BAP) as the plant growth regulators. It was observed that the C. odorata callus could be induced on media containing the combination of 3 mg/L NAA and 0.5 mg/L BAP. High concentration of NAA gave rise to pale, whitish and friable callus after 1 - 2 weeks of culture. The optimum pH for the cell culture was about 5.7 and incubation at 25 ± 2°C, in totally dark condition.
|Keyword:||Cananga odorata; 1-naphtalene acetic acid; 6-benzylaminopurine; Callus culture|
|Subject:||Cell culture - Laboratory manuals|
|Faculty or Institute:||Faculty of Biotechnology and Biomolecular Sciences|
|Deposited By:||Nida Hidayati Ghazali|
|Deposited On:||16 Aug 2012 07:59|
|Last Modified:||02 Nov 2012 01:12|
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