Cytogenetic Studies On The Water Buffalo (Bubalus Bubalis)
Abdullah, Mohamad Hilmi (1984) Cytogenetic Studies On The Water Buffalo (Bubalus Bubalis). PhD thesis, Universiti Pertanian Malaysia.
Chromosome analysis was undertaken on Murrah,swamp , F1 (Murrah x swamp ) and backcross (F1 female x Murrah male) buffaloes (Bubalus bubalis) using a modified leucocyte culture method combined with Giemsa (G) constitutive heterochromatin (C) and nucleolus organiser region (NOR) banding procedures . A combination of 80% medium RPMI 1640 , 20% calf serum , pokeweed mitogen and buffy coat gave many good quality metaphases . The Murrah , swamp and F1 buffaloes had chromosome complement s of 2n = 50 , 2n = 48 and 2n = 49 respectively . The Murrah karyotype had 5 pairs of submetacentric chromosome s and 20 pairs of acrocentric chromosomes while the swamp karyotype had 1 pair of large metacentric chromosomes , 4 submetacentric pairs and 19 pairs of acrocentric chromosomes. /F1 hybrid had an intermediate karyotype. Nine backcross animals had a 2n = 49 complement while 3 backcross animals had a 2n = 50 complement.
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