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Cytogenetic Studies On The Water Buffalo (Bubalus Bubalis)

Abdullah, Mohamad Hilmi (1984) Cytogenetic Studies On The Water Buffalo (Bubalus Bubalis). PhD thesis, Universiti Pertanian Malaysia.

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Abstract

Chromosome analysis was undertaken on Murrah,swamp , F1 (Murrah x swamp ) and backcross (F1 female x Murrah male) buffaloes (Bubalus bubalis) using a modified leucocyte culture method combined with Giemsa (G) constitutive heterochromatin (C) and nucleolus organiser region (NOR) banding procedures . A combination of 80% medium RPMI 1640 , 20% calf serum , pokeweed mitogen and buffy coat gave many good quality metaphases . The Murrah , swamp and F1 buffaloes had chromosome complement s of 2n = 50 , 2n = 48 and 2n = 49 respectively . The Murrah karyotype had 5 pairs of submetacentric chromosome s and 20 pairs of acrocentric chromosomes while the swamp karyotype had 1 pair of large metacentric chromosomes , 4 submetacentric pairs and 19 pairs of acrocentric chromosomes. /F1 hybrid had an intermediate karyotype. Nine backcross animals had a 2n = 49 complement while 3 backcross animals had a 2n = 50 complement.

Item Type:Thesis (PhD)
Subject:Water buffalo - Cytogenetics.
Chairman Supervisor:Associate Professor Dr. Tuan Ariffeen Bongso
Call Number:FPV 1984 3
Faculty or Institute:Faculty of Veterinary Medicine
ID Code:12263
Deposited By: Mohd Nezeri Mohamad
Deposited On:13 Jul 2011 15:35
Last Modified:03 Sep 2012 10:57

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