Abdullah, Mohamad Hilmi (1984) Cytogenetic Studies On The Water Buffalo (Bubalus Bubalis). PhD thesis, Universiti Pertanian Malaysia.
Chromosome analysis was undertaken on Murrah,swamp , F1 (Murrah x swamp ) and backcross (F1 female x Murrah male) buffaloes (Bubalus bubalis) using a modified leucocyte culture method combined with Giemsa (G) constitutive heterochromatin (C) and nucleolus organiser region (NOR) banding procedures . A combination of 80% medium RPMI 1640 , 20% calf serum , pokeweed mitogen and buffy coat gave many good quality metaphases . The Murrah , swamp and F1 buffaloes had chromosome complement s of 2n = 50 , 2n = 48 and 2n = 49 respectively . The Murrah karyotype had 5 pairs of submetacentric chromosome s and 20 pairs of acrocentric chromosomes while the swamp karyotype had 1 pair of large metacentric chromosomes , 4 submetacentric pairs and 19 pairs of acrocentric chromosomes. /F1 hybrid had an intermediate karyotype. Nine backcross animals had a 2n = 49 complement while 3 backcross animals had a 2n = 50 complement.
|Item Type:||Thesis (PhD)|
|Subject:||Water buffalo - Cytogenetics.|
|Chairman Supervisor:||Associate Professor Dr. Tuan Ariffeen Bongso|
|Call Number:||FPV 1984 3|
|Faculty or Institute:||Faculty of Veterinary Medicine|
|Deposited By:||Mohd Nezeri Mohamad|
|Deposited On:||13 Jul 2011 07:35|
|Last Modified:||03 Sep 2012 02:57|
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