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Vibriosis Vaccine Development: Pathogenesis, Immunological And Molecular Characterization Of Vibrio Alginolyticus

Mohd Taib, Nor Azizah (2000) Vibriosis Vaccine Development: Pathogenesis, Immunological And Molecular Characterization Of Vibrio Alginolyticus. Masters thesis, Universiti Putra Malaysia.

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Abstract

The aim of the research is to develop an effective vaccine against vibriosis. Vibriosis is a bacterial disease caused by Vibrio spp due to the intensive production activity of brackishwater ponds and cage-cultured fish. The pathogenicity study of V alginolyticus was performed by challenging the juvenile seabass (10±0.75 g) with 5 different isolates of V alginolyticus at different cell concentrations. All the isolates caused mortality to fish at a concentration as low as 0.2 optical density (6.28 X 103 CFU/ml). Ultrastructure changes observed by scanning and transmission electron microscopy, revealed the presence of V .alginolyticus in the gills, liver, muscle, spleen and kidney of infected fish. In addition, the V .alginolyticus cells were observed in the spleen. These pathological changes showed that V alginolyticus was responsible for the death of the infected seabass.

Item Type:Thesis (Masters)
Chairman Supervisor:Mariana Nor ht. Shamsudin, PhD
Call Number:FPSK(M) 2001 1
Faculty or Institute:Faculty of Medicine and Health Science
ID Code:11211
Deposited By: Mohd Nezeri Mohamad
Deposited On:18 Jul 2011 04:52
Last Modified:08 Aug 2011 05:08

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