Enhancement of Biocontrol Activities of Trichoderma Harzianum Rifai Through Protoplast Fusion

Wong, Mui Yun (1999) Enhancement of Biocontrol Activities of Trichoderma Harzianum Rifai Through Protoplast Fusion. Masters thesis, Universiti Putra Malaysia.

[img] PDF
1623Kb

Abstract

Enhancement of the biocontrol activities of Trichoderma harzianum Rifai against two soilborne pathogens, Sclerotium rolfsii and Ganoderma boninense through protoplast fusion was attempted. Mycelial cultures from three indigenous isolates from the rhizospheres of groundnut (IMI 378843), chilli (IMI 378844) and oil palm (IMI 378841) were used for the protoplast isolation and fusion studies. The result showed that the optimum release of viable protoplasts was obtained when mycelial cultures at the exponential stage was incubated for 4 h in Novozym 234 (Sigma) as the lytic enzyme at concentration of 7 mg/ml dissolved in 0.7 M NaCI and 0.6 M sorbitol. Pretreatment of mycelium v.ith 0.01 M 2-mercaptoethanol gave no Significant difference on protoplast yield of the three isolates studied. The protoplast yield was within the range of 10⁶-10⁸ protoplasts/ml and the average size of the protoplasts was 2.5-10.0 µm. Chemically induced fusion, using polyethylene glycol (pEG), among the three isolates yielded a total of 12 fusants. germinated 18 h after incubation in liquid Protoplast Regeneration Medium (PRM). When plated on solid PRM, the fusants regenerated into single colonies between 24-48 h after incubation. Of the 12 fusants obtained, five fusants showed non-parental type in isozyme analysis. They were further evaluated based on the cultural and morphological analysis, biomass growth, antagonistic activities against S. Tolfsii and G. boninense, tolerance to commonly used fungicides, and the ability to produce two extracellular lytic enzymes, B-l,3-glucanase and chitinase.

Item Type:Thesis (Masters)
Subject:Trichoderma
Chairman Supervisor:Associate Professor Dr. Sariah Meon
Call Number:FP 1999 2
Faculty or Institute:Faculty of Agriculture
ID Code:10423
Deposited By: Mohd Nezeri Mohamad
Deposited On:11 Apr 2011 02:37
Last Modified:11 Apr 2011 02:40

Repository Staff Only: Edit item detail

Document Download Statistics

This item has been downloaded for since 11 Apr 2011 02:37.

View statistics for "Enhancement of Biocontrol Activities of Trichoderma Harzianum Rifai Through Protoplast Fusion"


Universiti Putra Malaysia Institutional Repository

Universiti Putra Malaysia Institutional Repository is an on-line digital archive that serves as a central collection and storage of scientific information and research at the Universiti Putra Malaysia.

Currently, the collections deposited in the IR consists of Master and PhD theses, Master and PhD Project Report, Journal Articles, Journal Bulletins, Conference Papers, UPM News, Newspaper Cuttings, Patents and Inaugural Lectures.

As the policy of the university does not permit users to view thesis in full text, access is only given to the first 24 pages only.