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Interleukin-27 disrupts the crosstalk of apoptotic activities between 4T1 breast cancer cells and M2 macrophages


Citation

Mohd Yusof, Nurliyana and Mohamed Noor Fuadi, Natasha Nurafiqah and Hamid, Muhajir and Mohammed Alitheen, Noorjahan Banu and Mohd Hanafi, Nursyuhaida and Nik Abd Rahman, Nik Mohd Afizan (2022) Interleukin-27 disrupts the crosstalk of apoptotic activities between 4T1 breast cancer cells and M2 macrophages. Malaysian Journal of Medicine and Health Sciences, 18 (6). pp. 125-133. ISSN 2636-9346

Abstract

Introduction: Cytokine immunotherapy such as Interleukin-27 (IL-27) has been foreseen as a promising alternative anti-cancer treatment. Thus, this study aimed to investigate whether IL-27 gene therapy regulates crosstalk between breast cancer cells and macrophages in the sense of pro-apoptotic activities. Methods: This study has led to the development of recombinant pcDNA3.4-IL27. The recombinant pcDNA3.4-IL27 was transfected into 4T1 murine mammary carcinoma cells alone and co-culture of 4T1 with M2 macrophages. The successful expression of IL-27 in the cells were determine through the immunofluorescence staining and detection of CD206, M2 macrophages marker. Apoptotic effects of pcDNA3.4-IL27 were assessed through MTT assay, Annexin V flow cytometer analysis, and AO/PI dual staining. Results: Our findings shows that pcDNA3.4-IL27 has the ability to induce apoptosis in both of the cell group and performs better in the co-culture of 4T1 with M2 macrophages compared to 4T1 cells alone. PcDNA3.4-IL27 induced apoptosis through the altered cell morphology and reduction in the number of viable cells. Conclusion: These data demonstrate that pcDNA3.4-IL27 has the ability to induce apoptosis in both 4T1 cell alone and co-cultured 4T1 with M2 macrophages. Thus, could serve as a potential anti cancer candidate against breast cancer.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Institute of Tropical Forestry and Forest Products
DOI Number: https://doi.org/10.47836/mjmhs18.6.18
Publisher: Faculty of Medicine and Health Sciences, Universiti Putra Malaysia
Keywords: Breast cancer; Interleukin-27 (IL-27); pcDNA3.4-IL27; apoptosis; M2 macrophages
Depositing User: Mohamad Jefri Mohamed Fauzi
Date Deposited: 13 Mar 2023 07:17
Last Modified: 13 Mar 2023 07:17
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.47836/mjmhs18.6.18
URI: http://psasir.upm.edu.my/id/eprint/99343
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