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Effect of vacuum impregnation with sucrose and plant growth hormones to mitigate the chilling injury in spinach leaves


Karim, Nazatul Umira and Yusof, Noor Liyana (2021) Effect of vacuum impregnation with sucrose and plant growth hormones to mitigate the chilling injury in spinach leaves. Applied Sciences-Basel, 11 (21). art. no. 10410. pp. 1-15. ISSN 2076-3417


Vacuum impregnation (VI) has been immensely used in modifying the physicochemical properties, nutritional values and sensory attributes of fruits and vegetables. However, the metabolic consequences of the plant tissue upon impregnation have not been profoundly explored although shelf life is strongly dependent on this factor. In this study, spinach leaves were impregnated with salicylic acid (SA), γ-aminobutyric acid (GABA) and sucrose to improve its quality and storage ability by reducing the chilling injury through the improvement of proline content. The spinach leaves were stored at 4 °C for 7 days and were analyzed at 12 h interval. Upon 1 day of impregnation, the proline content in GABA, sucrose and SA impregnated leaves was increased by 240%, 153% and 103%, respectively, while in non-impregnated leaves, the proline content was decreased by 23.8%. The chlorophyll content of GABA impregnated leaves exhibited the lowest reduction (49%) followed by sucrose (55%) and SA (57%); meanwhile, non-impregnated leaves reduced 80% of chlorophyll content at the end of storage. Sensory evaluation showed that GABA, sucrose and SA impregnated leaves respectively, obtained higher score in terms of freshness, color, texture and overall appearance as compared to non-impregnated leaves.

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Additional Metadata

Item Type: Article
Divisions: Faculty of Food Science and Technology
Halal Products Research Institute
DOI Number: https://doi.org/10.3390/app112110410
Publisher: Multidisciplinary Digital Publishing Institute
Keywords: Physicochemical changes; Metabolic consequences; Proline; GABA; Shelf life
Depositing User: Ms. Nuraida Ibrahim
Date Deposited: 19 Apr 2023 04:07
Last Modified: 19 Apr 2023 04:07
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.3390/app112110410
URI: http://psasir.upm.edu.my/id/eprint/96954
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