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Antimicrobial activity and mode of action of 1,8-cineol against carbapenemase-producing Klebsiella pneumoniae


Moo, Chew Li and Osman, Mohd Azuraidi and Shun, Kai Yang and Wai, Sum Yap and Ismail, Saila and Swee, Hua Erin Lim and Chong, Chou Min and Lai, Kok Song (2021) Antimicrobial activity and mode of action of 1,8-cineol against carbapenemase-producing Klebsiella pneumoniae. Scientific Reports, 11. art. no. 20824. pp. 1-22. ISSN 2045-2322


Antimicrobial resistance remains one of the most challenging issues that threatens the health of people around the world. Plant-derived natural compounds have received considerable attention for their potential role to mitigate antibiotic resistance. This study was carried out to assess the antimicrobial activity and mode of action of a monoterpene, 1,8-cineol (CN) against carbapenemase-producing Klebsiella pneumoniae (KPC-KP). Results showed that resazurin microplate assay and time-kill analysis revealed bactericidal effects of CN at 28.83 mg/mL. Zeta potential showed that CN increased the surface charge of bacteria and an increase of outer membrane permeability was also detected. CN was able to cause leakage of proteins and nucleic acids in KPC-KP cells upon exposure to CN and ethidium bromide influx/efflux experiment showed the uptake of ethidium bromide into the cell; this was attributed to membrane damage. CN was also found to induce oxidative stress in CN-treated KPC-KP cells through generation of reactive oxygen species which initiated lipid peroxidation and thus damaging the bacterial cell membrane. Scanning and transmission electron microscopies further confirmed the disruption of bacterial cell membrane and loss of intracellular materials. In this study, we demonstrated that CN induced oxidative stress and membrane damage resulting in KPC-KP cell death.

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Official URL or Download Paper: https://www.nature.com/articles/s41598-021-00249-y

Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Institute of Bioscience
Publisher: Nature Research
Keywords: Klebsiella pneumoniae; Respiratory; Infection; Human health
Depositing User: Ms. Nuraida Ibrahim
Date Deposited: 17 Mar 2023 08:29
Last Modified: 17 Mar 2023 08:29
URI: http://psasir.upm.edu.my/id/eprint/95915
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