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Optimizing conditions for microwave-assisted extraction of polyphenolic content and antioxidant activity of Barleria lupulina Lindl.


Citation

Ismail Suhaimy, Noor Wahida and Abd Gani, Siti Salwa and Zaidan, Uswatun Hasanah and Halmi, Mohd Izuan Effendi and Bawon, Paiman (2021) Optimizing conditions for microwave-assisted extraction of polyphenolic content and antioxidant activity of Barleria lupulina Lindl. Plants-Basel, 10 (4). art. no. 682. pp. 1-19. ISSN 2223-7747

Abstract

Barleria lupulina Lindl. (Acanthaceae) as an ornamental plant has been widely used in folklore medicine due to its abundancy in polyphenolic compounds. The present study examined conditions for optimal extraction of antioxidants from B. lupulina leaf extracts by using the microwave-assisted extraction (MAE) method. The effects of ethanol concentrations, microwave power, and extraction time on total phenolic content (TPC), total flavonoid content (TFC), 1-diphenyl2-picrylhydrazyl (DPPH), and 2,20-azino-bis (3-ethylbenzothizoline-6-sulfonic acid) (ABTS) were investigated by single-factor experiments. Response surface methodology (RSM) was applied to observe interactions of three independent variables (ethanol concentrations, microwave power, and extraction time) on the dependent variables (TPC, TFC, DPPH, and ABTS) to establish optimal extraction conditions. Quadratic polynomial equations in all experimental models yielded favorably with fitted models with R2 and R2 adj of more than 0.90 and a non-significant lack of fit at p > 0.05. The optimal conditions for the extraction of antioxidant activity were established at 80% (v/v) ethanol, 400 W, and 30 s with TPC (238.71 mg gallic acid equivalent (GAE)/g sample), TFC (58.09 mg QE/g sample), DPPH (87.95%), and ABTS (89.56%). Analysis by ultra-high-performance liquid chromatography– quadrupole time-of-flight mass spectrometry (UHPLC-QTOF/MS) successfully identified four new phenylethanoid glycoside compounds in the species.


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Official URL or Download Paper: https://www.mdpi.com/2223-7747/10/4/682

Additional Metadata

Item Type: Article
Divisions: Faculty of Agriculture
Faculty of Biotechnology and Biomolecular Sciences
Faculty of Forestry
Halal Products Research Institute
DOI Number: https://doi.org/10.3390/plants10040682
Publisher: MDPI
Keywords: Barleria lupulina Lindl.; Microwave-assisted extraction (MAE); Response surface methodology (RSM); Total flavonoid content (TFC); Total phenolic content (TPC); 1-diphenyl-2-picrylhydrazyl (DPPH); 2,20-azino-bis (3-ethylbenzothizoline-6-sulfonic acid) (ABTS)
Depositing User: Ms. Nur Faseha Mohd Kadim
Date Deposited: 07 Feb 2023 03:54
Last Modified: 07 Feb 2023 03:54
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.3390/plants10040682
URI: http://psasir.upm.edu.my/id/eprint/94468
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