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Utilization of glucose recovered by phase separation system from acid-hydrolysed oil palm empty fruit bunch for bioethanol production


Mohd Zainudin, Mohd Huzairi and Abdul Rahman, Nor'aini and Abd. Aziz, Suraini and Funaoka, Masamitsu and Shinano, Takanori and Shirai, Yoshihito and Wakisaka, Minato and Hassan, Mohd Ali (2012) Utilization of glucose recovered by phase separation system from acid-hydrolysed oil palm empty fruit bunch for bioethanol production. Pertanika Journal of Tropical Agricultural Science, 35 (1). pp. 117-126. ISSN 1511-3701; ESSN: 2231-8542


Oil palm empty fruit bunch (OPEFB) is one the most abundant lignocellulosic wastes produced throughoutthe year in the palm oil industry. A new process of separating lignocellulose components after acid hydrolysis(known as phase separation system) has been previously developed, by which lignin and carbohydrate can becompletely and rapidly separated in 60 minutes between 25 and 30°C. In this process, cellulose is completelyhydrolyzed to oligosaccharides and remains in the acid phase. The maximum glucose yield of 53.8% wasobtained by hydrolysis, with 4% acid after autoclaving at 121°C for 5 minutes. This work focused on theseparation of monosaccharide (glucose) from cellulose fraction, which was subsequently used as a substratefor ethanol production. For this purpose, different types of nitrogen sources were evaluated, with yeast extractas the best nitrogen source (93% of theoretical yield) as compared to palm oil mill effluent (POME) andsludge powder for the growth of acid tolerant Saccharomyces cerevisiae ATCC 26602. Batch and repeatedbatch fermentation of S. cerevisiae ATCC 26602 using OPEFB hydrolysate gave 0.46 g glucose g ethanol-1,representing 87% of theoretical yield with a productivity of about 0.82 g-1 l-1 h-1 and 0.48 g glucose g ethanol-1,representing 89% of theoretical yield with productivity of about 2.79 g-1 l-1 h-1, respectively.

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Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Publisher: Universiti Putra Malaysia Press
Keywords: Bioethanol; Oil palm empty fruit bunch; Phase separation system; Acid hydrolysis; Glucose
Depositing User: Muizzudin Kaspol
Date Deposited: 03 Nov 2015 04:24
Last Modified: 02 Sep 2016 07:48
URI: http://psasir.upm.edu.my/id/eprint/944
Statistic Details: View Download Statistic

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