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Loop-mediated isothermal amplification (LAMP) as a promising point-of-care diagnostic strategy in avian virus research


Padzil, Faiz and Abdul Razak, Mariatulqabtiah and Tan, Wen Siang and Ho, Kok Lian and Mat Isa, Nurulfiza and Lau, Han Yih and Abu, Jalila and Chuang, Kuo-Pin (2021) Loop-mediated isothermal amplification (LAMP) as a promising point-of-care diagnostic strategy in avian virus research. Animals, 12 (1). art. no. 76. pp. 1-19. ISSN 2076-2615


Over the years, development of molecular diagnostics has evolved significantly in the detection of pathogens within humans and their surroundings. Researchers have discovered new species and strains of viruses, while mitigating the viral infections that occur, owing to the accessibility of nucleic acid screening methods such as polymerase chain reaction (PCR), quantitative (real-time) polymerase chain reaction (qPCR) and reverse-transcription qPCR (RT-qPCR). While such molecular detection methods are widely utilized as the benchmark, the invention of isothermal amplifications has also emerged as a reliable tool to improvise on-field diagnosis without dependence on thermocyclers. Among the established isothermal amplification technologies are loop-mediated isothermal amplification (LAMP), recombinant polymerase amplification (RPA), strand displacement activity (SDA), nucleic acid sequence-based amplification (NASBA), helicase-dependent amplification (HDA) and rolling circle amplification (RCA). This review highlights the past research on and future prospects of LAMP, its principles and applications as a promising point-of-care diagnostic method against avian viruses.

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Official URL or Download Paper: https://www.mdpi.com/2076-2615/12/1/76

Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Faculty of Medicine and Health Science
Faculty of Veterinary Medicine
Institute of Bioscience
DOI Number: https://doi.org/10.3390/ani12010076
Publisher: MDPI AG
Keywords: Avian; Virus; Diagnostic; LAMP; Rapid
Depositing User: Ms. Che Wa Zakaria
Date Deposited: 18 May 2023 02:24
Last Modified: 18 May 2023 02:24
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.3390/ani12010076
URI: http://psasir.upm.edu.my/id/eprint/94052
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