Simple Search:

Efficacy of an inactivated recombinant vaccine encoding a fimbrial protein of Pasteurella multocida B:2 against hemorrhagic septicemia in goats


Citation

Mohd Yasin, Ina Salwany and Mohd Yusoff, Sabri and Saad, Mohd Zamri and Abd Wahid, Mohd Effendy (2010) Efficacy of an inactivated recombinant vaccine encoding a fimbrial protein of Pasteurella multocida B:2 against hemorrhagic septicemia in goats. Tropical Animal Health and Production, 43 (1). pp. 179-187. ISSN 0049-4747

Abstract / Synopsis

This study was carried out to determine the antibody responses and protective capacity of an inactivated recombinant vaccine expressing the fimbrial protein of Pasteurella multocida B:2 following intranasal vaccination against hemorrhagic septicemia in goats. Goats were vaccinated intranasal with 106 CFU/mL of the recombinant vaccine (vaccinated group) and 106 CFU/mL of pET32/LIC vector without fimbrial protein (control group). All three groups were kept separated before all goats in the three groups were challenged with 109 CFU/mL of live pathogenic P. multocida B:2. During the course of study, both serum and lung lavage fluid were collected to evaluate the antibody levels via enzyme-linked immunosorbent assay. It was found that goats immunized with the inactivated recombinant vaccine developed a strong and significantly (p < 0.05) higher specific IgA and IgG responses in both serum and lung lavage fluid samples compared to the control and unvaccinated groups. Following intratracheal challenge, the rate of isolation was 17% for the vaccinated group, 67% for the control group and 100% for the unvaccinated group. However, none of the goat from the vaccinated group had P. multocida B:2 in the liver, tonsil and heart. Therefore, the study revealed that an inactivated recombinant vaccine significantly provides significant protection against high dose challenge and enhances the stimulation of the local and systemic immunities.


Download File

[img]
Preview
PDF (Abstract)
Efficacy of an inactivated recombinant vaccine encoding a fimbrial protein of Pasteurella multocida B.pdf

Download (184kB) | Preview

Additional Metadata

Item Type: Article
Divisions: Faculty of Veterinary Medicine
DOI Number: https://doi.org/10.1007/s11250-010-9672-5
Publisher: Springer Science + Business Media B.V. 2007
Keywords: Pasteurella multocida B:2, Inactivated recombinant vaccine, Intranasal, Goats
Depositing User: Azizan Arshad
Date Deposited: 28 Jan 2011 05:13
Last Modified: 20 Oct 2015 08:06
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1007/s11250-010-9672-5
URI: http://psasir.upm.edu.my/id/eprint/9368
Statistic Details: View Download Statistic

Actions (login required)

View Item View Item