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Lipase-Catalyzed Synthesis of Wax Ester


Mat Hadzir, Nursyamsyila (2001) Lipase-Catalyzed Synthesis of Wax Ester. Masters thesis, Universiti Putra Malaysia.


Alcoholysis reaction between triolein and oleyl alcohol catalyzed by Lipozyme and Novozyme was carried out to produce oleyl oleate, a wax ester. Several reaction parameters were studied in order to get its optimal condition such as effect of time, reaction temperature, amount of enzyme, molar ratio of substrates (oleyl alcohol/trioleib), various organic solvents used, initial water activity (aw), continuously controlled water activity and addition of silica gel. The optimal condition to produce wax ester for Lipozyme are incubation time of 5 h, temperature of 50°c and using 30% (w/v) of Lipozyme. The molar ratio of substmte was 6:1 (oleyl alcohol/triolein) in heptane and at aw 0.33 (initial water activity) or 0.32 (continuously controlled water activity) with the addition of 0.25 g of silica gel. For Novozyme, the optimum incubation time was 5 h, at reaction temperature of 60 °c using 30% (w/v) of Novozyme. The optimum molar ratio of substrates (oleyl alcohol/triolein) was also at 6:1, in heptane at aw 0.33 (initial water activity, aw), 0.32 (continuously controlled water activity) and with the addition of 0.50 g of silica gel. A scale up reaction was then carried out using the optimum condition catalyzed by Lipozyme. The percentage yield of wax ester produced at optimum reaction condition was 58.48% when determined by gravimetry method and 75.66% when determined using gas chromatography.

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Additional Metadata

Item Type: Thesis (Masters)
Subject: Lipase - Synthesis
Subject: Catalysts
Call Number: FSAS 2001 54
Chairman Supervisor: Associate Professor Dr. Mahiran Basri, PhD
Divisions: Faculty of Science and Environmental Studies
Depositing User: Tuan Norasiah Tuan Yaacob
Date Deposited: 21 Jan 2011 04:22
Last Modified: 30 Sep 2013 07:01
URI: http://psasir.upm.edu.my/id/eprint/9321
Statistic Details: View Download Statistic

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