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Acetylcholine receptor-based biosensor derived from Asian swamp eel, Monopterus albus for heavy metals biomonitoring


Muhammad Khalidi, Siti Aishah and Sabullah, Mohd Khalizan and Abdul Sani, Suraya and Abd. Shukor, Mohd Yunus and Basirun, Ain Aqilah and Abd Gafar, A’aishah and Mohd Jaafar, ‘Izazy Nur and Noordin, Noreen (2020) Acetylcholine receptor-based biosensor derived from Asian swamp eel, Monopterus albus for heavy metals biomonitoring. Pertanika Journal of Science & Technology, 28 (spec. 2). pp. 83-94. ISSN 0128-7680; ESSN: 2231-8526


Cholinesterase-based biosensor well known as a sensitive method to detect the existence of harmful dissolved compounds in any type of water source, especially the river. This alternative biosensor can be used to determine the level of pollution of the water in a short period of time as well as to evaluate the low cost and simple service. The aim of this study was to exceed the effectiveness of acetylcholinesterase source extracted from the brain tissue of Asian swamp eel; Monopterus albus as a potential environmental biosensor. Purified acetylcholinesterase exposed to a different type of metal ions and mercury showed the highest percentage of inhibition at 62.9% followed by chromium at 59.22% while silver, arsenic, cadmium, cobalt, copper, nickel, zinc and lead at not more than 50% (approximately 37-50%). Metal ions such as mercury, zinc, chromium and copper showed exponential decay type inhibition curves with calculated half maximal inhibitory concentration; IC50 in the ascending sensitivity order 0.005, 0.595, 0.687 and 1.329 mgL-1, respectively. Field trial works exhibited that the acetylcholinesterase was applicable in sensing heavy metals pollution from the river which closed to the industrial and agricultural sites at near real-time and verified using ICP-OES. This study proves the potential use of acetylcholinesterase sourced from M. albus as a biomonitoring tool to assess the contamination level of the river.

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Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Publisher: Universiti Putra Malaysia Press
Keywords: Acetylcholinesterase; Biosensor; Heavy metal; IC50; Monopterus albus
Depositing User: Mohamad Jefri Mohamed Fauzi
Date Deposited: 10 Sep 2021 09:45
Last Modified: 10 Sep 2021 09:45
URI: http://psasir.upm.edu.my/id/eprint/90434
Statistic Details: View Download Statistic

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