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Selection of acid salts: a critical step in creating an acidic condition for plasma iron release and measurement


Lim, Wai Feng and Abdullah, Maha and Ho, Kok Lian and Yap, Boon Kar and Lai, Mei I. (2020) Selection of acid salts: a critical step in creating an acidic condition for plasma iron release and measurement. Malaysian Journal of Medicine and Health Sciences, 16 (suppl. 9). pp. 52-57. ISSN 1675-8544; ESSN: 2636-9346


Introduction: Iron deficiency anaemia (IDA) is the most common cause of anaemia worldwide. Determination of body iron status is necessary to diagnose IDA. This can be measured using a biochemistry assessment of the serum/ plasma. Plasma/serum iron quantitation is also important in diagnosing iron overload disorders. However, iron studies are limited due to high cost and lack of access to biochemical analysers. Therefore, a cost- and technical-effective method is needed to measure human plasma iron concentration. Plasma iron is mainly transferrin-bound and an acidic plasmic condition is necessary to release the iron. This study investigated various candidate acid salts to achieve the acidic condition needed for plasma iron release. Method: Ten powdered or crystallised acid salts were studied for their water solubility as well as their pH reduction capability in revised simulated body fluid (r-SBF) and commercially available human plasma without any change in colour or form. Results: Six acid salts studied were discontinued from further investigation because they were insoluble in water. Another two candidates were unsuitable as they precipitated in r-SBF and human plasma. Maleic acid formed a jelly-like texture after a certain amount of time in human plasma. Only citric acid met all the criteria of a suitable acid salt to be investigated further as part of the reagent for a spontaneous plasma iron measurement. Conclusion: Citric acid, which is a colourless and odourless acid salt, was selected to lower the human plasma pH to an acidic condition for transferrin-bound iron release.

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Additional Metadata

Item Type: Article
Divisions: Faculty of Medicine and Health Science
Institute of Bioscience
Publisher: Faculty of Medicine and Health Sciences, Universiti Putra Malaysia
Keywords: Acid salts; Plasma iron; Citric acid; Iron deficiency anaemia; Human plasma
Depositing User: Mohamad Jefri Mohamed Fauzi
Date Deposited: 08 Sep 2021 22:37
Last Modified: 08 Sep 2021 22:41
URI: http://psasir.upm.edu.my/id/eprint/90190
Statistic Details: View Download Statistic

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