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l-ascorbic acid and thymoquinone dual-loaded palmitoyl-chitosan nanoparticles: improved preparation method, encapsulation and release efficiency


Othman, Nurhanisah and Md. Jamil, Siti Nurul Ain and Masarudin, Mas Jaffri and Abdullah, Luqman Chuah and Daik, Rusli and Sarman, Nor Syazwani (2020) l-ascorbic acid and thymoquinone dual-loaded palmitoyl-chitosan nanoparticles: improved preparation method, encapsulation and release efficiency. Processes, 8 (9). art. no. 1040. pp. 1-19. ISSN 2227-9717


Encapsulation of dual compounds of different characters (hydrophilic and hydrophobic) in single nanoparticles carrier could reach the site of action more accurately with the synergistic effect but it is less investigated. In our previous findings, combined-compounds encapsulation and delivery from chitosan nanoparticles were impaired by the hydrophilicity of chitosan. Therefore, hydrophobic modification on chitosan with palmitic acid was conducted in this study to provide an amphiphilic environment for better encapsulation of antioxidants; hydrophobic thymoquinone (TQ) and hydrophilic l-ascorbic acid (LAA). Palmitoyl chitosan nanoparticles (PCNPs) co-loaded with TQ and LAA (PCNP-TQ-LAA) were synthesized via the ionic gelation method. Few characterizations were conducted involving nanosizer, Fourier-transform infrared spectroscopy (FTIR), field-emission scanning electron microscopy (FESEM) and high-resolution transmission electron microscopy (HRTEM). UV–VIS spectrophotometry was used to analyze the encapsulation and release efficiency of the compounds in PCNPs. Successfully modified PCNP-TQ-LAA had an average particle size of 247.7 ± 24.0 nm, polydispersity index (PDI) of 0.348 ± 0.043 and zeta potential of 19.60 ± 1.27 mV. Encapsulation efficiency of TQ and LAA in PCNP-TQ-LAA increased to 64.9 ± 5.3% and 90.0 ± 0%, respectively. TQ and LAA in PCNP-TQ-LAA system showed zero-order release kinetics, with a release percentage of 97.5% and 36.1%, respectively. Improved preparation method, encapsulation and release efficiency in this study are anticipated to be beneficial for polymeric nanocarrier development.

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Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Faculty of Engineering
Faculty of Science
Institute of Bioscience
Centre of Foundation Studies for Agricultural Science
DOI Number: https://doi.org/10.3390/pr8091040
Publisher: Multidisciplinary Digital Publishing Institute
Keywords: Chitosan; Co-loaded nanoparticles; Hydrophobic modification; l-ascorbic acid; Thymoquinone
Depositing User: Ms. Nuraida Ibrahim
Date Deposited: 13 Aug 2021 23:38
Last Modified: 13 Aug 2021 23:38
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.3390/pr8091040
URI: http://psasir.upm.edu.my/id/eprint/89551
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