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Ion interaction and hydrogen bonds as main features of protein thermostability in mutated T1 recombinant lipase originated from Geobacillus zalihae


Citation

Ishak, Siti Nor Hasmah and Ahmad Kamarudin, Nor Hafizah and Mohamad Ali, Mohd Shukuri and Thean, Adam Chor Leow and Raja Abd. Rahman, Raja Noor Zaliha (2020) Ion interaction and hydrogen bonds as main features of protein thermostability in mutated T1 recombinant lipase originated from Geobacillus zalihae. Molecules, 25 (15). art. no. 3430. pp. 1-17. ISSN 1420-3049

Abstract

A comparative structure analysis between space- and an Earth-grown T1 recombinant lipase from Geobacillus zalihae had shown changes in the formation of hydrogen bonds and ion-pair interactions. Using the space-grown T1 lipase validated structure having incorporated said interactions, the recombinant T1 lipase was re-engineered to determine the changes brought by these interactions to the structure and stability of lipase. To understand the effects of mutation on T1 recombinant lipase, five mutants were developed from the structure of space-grown T1 lipase and biochemically characterized. The results demonstrate an increase in melting temperature up to 77.4 °C and 76.0 °C in E226D and D43E, respectively. Moreover, the mutated lipases D43E and E226D had additional hydrogen bonds and ion-pair interactions in their structures due to the improvement of stability, as observed in a longer half-life and an increased melting temperature. The biophysical study revealed differences in β-Sheet percentage between less stable (T118N) and other mutants. As a conclusion, the comparative analysis of the tertiary structure and specific residues associated with ion-pair interactions and hydrogen bonds could be significant in revealing the thermostability of an enzyme with industrial importance.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Centre of Foundation Studies for Agricultural Science
DOI Number: https://doi.org/10.3390/molecules25153430
Publisher: Multidisciplinary Digital Publishing Institute
Keywords: T1 lipase; Hydrogen bonds; Ion-pair interactions; Thermostability; Site-directed mutagenesis
Depositing User: Ms. Nuraida Ibrahim
Date Deposited: 18 Aug 2021 08:31
Last Modified: 18 Aug 2021 08:31
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.3390/molecules25153430
URI: http://psasir.upm.edu.my/id/eprint/89462
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