UPM Institutional Repository

Integrated Stirred-Tank Bioreactor with Internal Adsorption for the Removal of Ammonium to Enhance the Cultivation Performance of gdhA Derivative Pasteurella multocida B:2


Citation

Oslan, Siti Nur Hazwani and Tan, Joo Shun and Abbasiliasi, Sahar and Sulaiman, Ahmad Ziad and Saad, Mohd Zamri and Halim, Murni and Ariff, Arbakariya (2020) Integrated Stirred-Tank Bioreactor with Internal Adsorption for the Removal of Ammonium to Enhance the Cultivation Performance of gdhA Derivative Pasteurella multocida B:2. Molecules, 8 (11). pp. 1-15. ISSN 1420-3049

Abstract

Growth of mutant gdhA Pasteurella multocida B:2 was inhibited by the accumulation of a by-product, namely ammonium in the culture medium during fermentation. The removal of this by-product during the cultivation of mutant gdhA P. multocida B:2 in a 2 L stirred-tank bioreactor integrated with an internal column using cation-exchange adsorption resin for the improvement of cell viability was studied. Different types of bioreactor system (dispersed and internal) with resins were successfully used for ammonium removal at different agitation speeds. The cultivation in a bioreactor integrated with an internal column demonstrated a significant improvement in growth performance of mutant gdhA P. multocida B:2 (1.05 × 1011 cfu/mL), which was 1.6-fold and 8.4-fold as compared to cultivation with dispersed resin (7.2 × 1010 cfu/mL) and cultivation without resin (1.25 × 1010 cfu/mL), respectively. The accumulation of ammonium in culture medium without resin (801 mg/L) was 1.24-fold and 1.37-fold higher than culture with dispersed resin (642.50 mg/L) and culture in the bioreactor integrated with internal adsorption (586.50 mg/L), respectively. Results from this study demonstrated that cultivation in a bioreactor integrated with the internal adsorption column in order to remove ammonium could reduce the inhibitory effect of this by-product and improve the growth performance of mutant gdhA P. multocida B:2.


Download File

[img] Text (Abstract)
AMMO.pdf

Download (6kB)

Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Faculty of Veterinary Medicine
Halal Products Research Institute
DOI Number: https://doi.org/10.3390/microorganisms8111654
Publisher: Multidisciplinary Digital Publishing Institute
Keywords: Cation-exchange resin; Adsorption; Removal; Ammonium; Cell viability; Mutant gdhA P. multocida B:2
Depositing User: Ms. Nuraida Ibrahim
Date Deposited: 19 Aug 2021 08:29
Last Modified: 19 Aug 2021 08:29
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.3390/microorganisms8111654
URI: http://psasir.upm.edu.my/id/eprint/89413
Statistic Details: View Download Statistic

Actions (login required)

View Item View Item