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Induction, multiplication, and evaluation of antioxidant activity of Polyalthia bullata callus, a woody medicinal plant


Kamarul Zaman, Munirah Adibah and Mohamad Azzeme, Azzreena and Ramle, Illy Kamaliah and Normanshah, Nurfazlinyana and Ramli, Siti Nurhafizah and Shaharuddin, Noor Azmi and Ahmad, Syahida and Abdullah, Siti Nor Akmar (2020) Induction, multiplication, and evaluation of antioxidant activity of Polyalthia bullata callus, a woody medicinal plant. Plants-Basel, 9 (12). pp. 1-23. ISSN 2223-7747


Polyalthia bullata is an endangered medicinal plant species. Hence, establishment of P. bullata callus culture is hoped to assist in mass production of secondary metabolites. Leaf and midrib were explants for callus induction. Both of them were cultured on Murashige and Skoog (MS) and Woody Plant Medium (WPM) containing different types and concentrations of auxins (2,4-dichlorophenoxyacetic acid (2,4-D), α-naphthaleneacetic acid (NAA), picloram, and dicamba). The callus produced was further multiplied on MS and WPM supplemented with different concentrations of 2,4-D, NAA, picloram, dicamba, indole-3-acetic acid (IAA), and indole-3-butyric acid (IBA) media. The quantification of total phenolic content (TPC), total flavonoid content (TFC) and antioxidant capacity was further carried out on P. bullata callus, and the results were subjected to correlation analysis. Among the media, the WPM + 16.56 µM picloram (53.33 ± 22.06%) was the best for callus induction while MS + 30 µM dicamba was the best for callus multiplication. The TPC, TFC, and EC50 of DPPH scavenging activity were determined at 0.657 ± 0.07 mg GAE/g FW, 0.491 ± 0.03 mg QE/g, and 85.59 ± 6.09 µg/mL in P. bullata callus, respectively. The positive correlation between DPPH scavenging activity with TPC was determined at r = 0.869, and that of TFC was at r = 0.904. Hence, the P. bullata callus has an ability to accumulate antioxidants. It therefore can be a medium for secondary metabolites production.

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Additional Metadata

Item Type: Article
Divisions: Faculty of Agriculture
Faculty of Biotechnology and Biomolecular Sciences
Institute of Plantation Studies
DOI Number: https://doi.org/10.3390/plants9121772
Publisher: Multidisciplinary Digital Publishing Institute
Keywords: Polyalthia bullata; Callus induction; Callus multiplication; Auxins; Total phenolic content; Total flavonoid content; Antioxidant activity
Depositing User: Ms. Nuraida Ibrahim
Date Deposited: 03 Sep 2021 05:44
Last Modified: 03 Sep 2021 05:44
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.3390/plants9121772
URI: http://psasir.upm.edu.my/id/eprint/89348
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