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Immunological analysis of the Hepatitis B virus “a” determinant displayed on chimeric virus-like particles of Macrobrachium rosenbergii nodavirus capsid protein produced in Sf9 cells


Citation

Ninyio, Nathaniel Nyakaat and Ho, Kok Lian and Ong, Hui Kian and Yong, Chean Yeah and Chee, Hui Yee and Hamid, Muhajir and Tan, Wen Siang (2020) Immunological analysis of the Hepatitis B virus “a” determinant displayed on chimeric virus-like particles of Macrobrachium rosenbergii nodavirus capsid protein produced in Sf9 cells. Vaccines, 8 (2). art. no. 275. pp. 1-15. ISSN 2076-393X

Abstract

Chimeric virus-like particles (VLPs) have been widely exploited for various purposes including their use as vaccine candidates, particularly due to their ability to induce stronger immune responses than VLPs consisting of single viral proteins. In the present study, VLPs of the Macrobrachium rosenbergii nodavirus (MrNV) capsid protein (Nc) displaying the hepatitis B virus “a” determinant (aD) were produced in Spodoptera frugiperda (Sf9) insect cells. BALB/c mice immunised with the purified chimeric Nc-aD VLPs elicited a sustained titre of anti-aD antibody, which was significantly higher than that elicited by a commercially available hepatitis B vaccine and Escherichia coli-produced Nc-aD VLPs. Immunophenotyping showed that the Sf9-produced Nc-aD VLPs induced proliferation of cytotoxic T-lymphocytes and NK1.1 natural killer cells. Furthermore, enzyme-linked immunospot (ELISPOT)analysis showed the presence of antibody-secreting memory B cells in the mice splenocytes stimulated with the synthetic aD peptide. The significant humoral, natural killer cell and memory B cell immune responses induced by the Sf9-produced Nc-aD VLPs suggest that they present good prospects for use as a hepatitis B vaccine candidate.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Faculty of Medicine and Health Science
Institute of Bioscience
DOI Number: https://doi.org/10.3390/vaccines8020275
Publisher: Multidisciplinary Digital Publishing Institute
Keywords: Vvirus-like particles; Sf9 cells; Hepatitis B; Macrobrachium rosenbergii nodavirus; Cytotoxic T-lymphocytes; Natural killer cells; Memory B cells; BALB/c mice
Depositing User: Ms. Nuraida Ibrahim
Date Deposited: 20 Sep 2021 22:58
Last Modified: 20 Sep 2021 22:58
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.3390/vaccines8020275
URI: http://psasir.upm.edu.my/id/eprint/89272
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