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Identification and analysis of microRNAs in Chlorella sorokiniana using high-throughput sequencing


Azaman, Siti Nor Ani and Satharasinghe, Dilan Amila and Tan, Sheau Wei and Nagao, Norio and Md Yusoff, Fatimah and Yeap, Swee Keong (2020) Identification and analysis of microRNAs in Chlorella sorokiniana using high-throughput sequencing. Genes, 11 (10). pp. 1-12. ISSN 2073-4425


Chlorella is a popular microalga with robust physiological and biochemical characteristics, which can be cultured under various conditions. The exploration of the small RNA content of Chlorella could improve strategies for the enhancement of metabolite production from this microalga. In this study, stress was introduced to the Chlorella sorokiniana culture to produce high-value metabolites such as carotenoids and phenolic content. The small RNA transcriptome of C. sorokiniana was sequenced, focusing on microRNA (miRNA) content. From the analysis, 98 miRNAs were identified in cultures subjected to normal and stress conditions. The functional analysis result showed that the miRNA targets found were most often involved in the biosynthesis of secondary metabolites, followed by protein metabolism, cell cycle, and porphyrin and chlorophyll metabolism. Furthermore, the biosynthesis of secondary metabolites such as carotenoids, terpenoids, and lipids was found mostly in stress conditions. These results may help to improve our understanding of regulatory mechanisms of miRNA in the biological and metabolic process of Chlorella species. It is important and timely to determine the true potential of this microalga species and to support the potential for genetic engineering of microalgae as they receive increasing focus for their development as an alternative source of biofuel, food, and health supplements.

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Official URL or Download Paper: https://www.mdpi.com/2073-4425/11/10/1131

Additional Metadata

Item Type: Article
Divisions: Faculty of Agriculture
Institute of Bioscience
Centre of Foundation Studies for Agricultural Science
DOI Number: https://doi.org/10.3390/genes11101131
Publisher: Multidisciplinary Digital Publishing Institute
Keywords: Chlorella sorokiniana; MiRNA; Metabolites; Transcription regulation
Depositing User: Ms. Nuraida Ibrahim
Date Deposited: 03 Sep 2021 08:51
Last Modified: 03 Sep 2021 08:51
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.3390/genes11101131
URI: http://psasir.upm.edu.my/id/eprint/89251
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