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Genome-wide identification and expression analysis of banana RBOH genes in response to Fusarium oxysporum f. sp. cubense Tropical Race 4


Citation

Chai, Siew Ying and Tahir, Nur Fathiah and Abu Bakar, Nur Syazana and Teo, Chee How and Mohd Yusoff, Nur Fatihah and Baharum, Nadiya Akmal and Saidi, Noor Baity (2020) Genome-wide identification and expression analysis of banana RBOH genes in response to Fusarium oxysporum f. sp. cubense Tropical Race 4. Malaysian Journal of Biochemistry and Molecular Biology, 23 (2). 46 - 52. ISSN 1511-2616

Abstract

Respiratory burst oxidase homologs (RBOH) is the key enzyme responsible for the production of reactive oxygen species which act as important signal during plant responses to abiotic and biotic stresses. However, RBOH homologs have not been characterized in banana. In this study, we have identified twelve Rboh genes distributed on eight chromosomes of Musa acuminata subsp. malaccensis (DH Pahang) through a genome-wide analysis. MaRbohs exist as sibling paralogs with variable exon-intron structures and highly conserved functional domain. Phylogenetic analysis clustered MaRBOH into four distinct subgroups (I, II, III and IV). The expression of MaRbohs following 24 hours of inoculation with Fusarium oxysporium f. sp. cubense Tropical Race 4 revealed diverse patterns in root tissues. MaRbohH1 and MaRbohA2 were strongly upregulated and downregulated, respectively in response to the root-dip inoculation. This is the first report on genome-wide characterization of Rboh genes from banana and their expression in response to the fungal pathogen TR4. This research provides a basis for exploration of the role of NADPH oxidase in banana defence against the root pathogen.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Publisher: Malaysian Society for Biochemistry and Molecular Biology
Keywords: Respiratory burst oxidase homologs (Rbohs); NADPH oxidase; Musa acuminata cv. Berangan; Fusarium oxysporum f. sp. cubense Tropical Race 4 (TR4)
Depositing User: Ms. Nuraida Ibrahim
Date Deposited: 07 Oct 2021 04:04
Last Modified: 07 Oct 2021 04:04
URI: http://psasir.upm.edu.my/id/eprint/89116
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