UPM Institutional Repository

Expression of genes encoding manganese peroxidase and laccase of Ganoderma boninense in response to nitrogen sources, hydrogen peroxide and phytohormones


Citation

Ho, Pei Yin and Namasivayam, Parameswari and Sundram, Shamala and Ho, Chai Ling (2020) Expression of genes encoding manganese peroxidase and laccase of Ganoderma boninense in response to nitrogen sources, hydrogen peroxide and phytohormones. Genes, 11 (11). pp. 1-15. ISSN 2073-4425

Abstract

Ganoderma produces lignolytic enzymes that can degrade the lignin component of plant cell walls, causing basal stem rot to oil palms. Nitrogen sources may affect plant tolerance to root pathogens while hydrogen peroxide (H2O2), salicylic acid (SA) and jasmonic acid (JA) play important roles in plant defense against pathogens. In this study, we examined the expression of genes encoding manganese peroxidase (MnP) and laccase (Lac) in Ganoderma boninense treated with different nitrogen sources (ammonium nitrate, ammonium sulphate, sodium nitrate and potassium nitrate), JA, SA and H2O2. Transcripts encoding MnP and Lac were cloned from G. boninense. Of the three GbMnP genes, GbMnP_U6011 was up-regulated by all nitrogen sources examined and H2O2 but was down-regulated by JA. The expression of GbMnP_U87 was only up-regulated by JA while GbMnP_35959 was up-regulated by ammonium nitrate but suppressed by sodium nitrate and down-regulated by H2O2. Among the three GbLac genes examined, GbLac_U90667 was up-regulated by ammonium nitrate, JA, SA and H2O2; GbLac_U36023 was up-regulated by JA and H2O2 while GbLac_U30636 was up-regulated by SA but suppressed by ammonium sulphate, sodium nitrate, JA and H2O2. Differential expression of these genes may be required by their different functional roles in G. boninense.


Download File

[img] Text (Abstract)
GANO.pdf

Download (70kB)

Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Institute of Plantation Studies
DOI Number: https://doi.org/10.3390/genes11111263
Publisher: Multidisciplinary Digital Publishing Institute
Keywords: Ganoderma; Hydrogen peroxide; Laccase; Manganese peroxidase; Nitrogen; Phytohormones
Depositing User: Ms. Nuraida Ibrahim
Date Deposited: 04 Oct 2021 22:23
Last Modified: 04 Oct 2021 22:23
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.3390/genes11111263
URI: http://psasir.upm.edu.my/id/eprint/88932
Statistic Details: View Download Statistic

Actions (login required)

View Item View Item