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Enzyme catalyzed esterification of sugar by thermostable t1 lipase from geobacillus zalihae in ionic liquid


Abdulmalek, Emilia and Mohd Saupi, Hanim Salami and Zulkefli, Syarilaida and Raja Abd Rahman, Raja Noor Zaliha and Abdul Rahman, Mohd Basyaruddin (2020) Enzyme catalyzed esterification of sugar by thermostable t1 lipase from geobacillus zalihae in ionic liquid. Malaysian Journal of Analytical Sciences, 24 (2). 188 - 196. ISSN 1394-2506


Thermostable T1 lipase from Geobacillus zalihae strain was utilized in esterification of various sugars with fatty acids to form fatty acid sugar esters. Fatty acid sugar esters (FASE) are an important class of non-ionic surfactant which possesses good emulsifying, stabilizing and conditioning properties. In this work the esterification reaction was done in [Bmim][BF4] ionic liquid with dimethylsulfoxide (DMSO) as co-solvent. Esterification of galactose with oleic acid was initially screened by varying the conditions (temperature, time, enzyme loading and the fatty acid chain length). It was found that the reaction was optimum at the following conditions: temperature (65 °C), time (120 minutes) and enzyme loading (2% (w/w)) when carried out in mixture of DMSO: [Bmim][BF4] (1:20). Interestingly, the percentage of conversion was not affected by the chain length of the acyl donor (C8 – C18) or the unsaturation degree. When the reaction was repeated with linoleic acid as acyl donor and different acyl acceptor (galactose, glucose, fructose, sucrose, maltose, trehalose and xylitol), sucrose gave the best conversion at 65%. In conclusion, TI lipase showed broad substrate specificity either for the acyl donor or acyl acceptor during the esterification of sugars in ionic liquid.

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Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Faculty of Science
Publisher: Faculty of Science and Technology, Universiti Kebangsaan Malaysia
Keywords: T1 lipase; Fatty acid sugar ester; Ionic liquid; Esterification; [Bmim][BF4]
Depositing User: Ms. Nuraida Ibrahim
Date Deposited: 02 Nov 2021 20:26
Last Modified: 02 Nov 2021 20:26
URI: http://psasir.upm.edu.my/id/eprint/88836
Statistic Details: View Download Statistic

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