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Enhancement of β-mannanase production by Bacillus subtilis ATCC11774 through optimization of medium composition


Mahamad Norizan, Nor Amalina and Halim, Murni and Tan, Joo Shun and Abbasiliasi, Sahar and Mat Sahri, Miskandar and Othman, Firdaus and Ariff, Arbakariya (2020) Enhancement of β-mannanase production by Bacillus subtilis ATCC11774 through optimization of medium composition. Molecules, 25 (15). pp. 1-4. ISSN 1420-3049


Palm kernel cake (PKC) has been largely produced in Malaysia as one of the cheap and abundant agro-waste by-products from the palm oil industry and it contains high fiber (mannan) content. The present study aimed to produce β-mannanase by Bacillus subtilis ATCC11774 via optimization of the medium composition using palm kernel cake as substrate in semi-solid fermentation. The fermentation nutrients such as PKC, peptone, yeast extract, sodium chloride, magnesium sulphate (MgSO2), initial culture pH and temperature were screened using a Plackett-Burman design. The three most significant factors identified, PKC, peptone and NaCl, were further optimized using central composite design (CCD), a response surface methodology (RSM) approach, where yeast extract and MgSO2 were fixed as a constant factor. The maximum β-mannanase activity predicted by CCD under the optimum medium composition of 16.50 g/L PKC, 19.59 g/L peptone, 3.00 g/L yeast extract, 2.72 g/L NaCl and 0.2 g/L MgSO2 was 799 U/mL. The validated β-mannanase activity was 805.12 U/mL, which was close to the predicted β-mannanas activity. As a comparison, commercial media such as nutrient broth, M9 and Luria bertani were used for the production of β-mannanase with activities achieved at 204.16 ± 9.21 U/mL, 50.32 U/mL and 88.90 U/mL, respectively. The optimized PKC fermentation medium was four times higher than nutrient broth. Hence, it could be a potential fermentation substrate for the production of β-mannanase activity by Bacillus subtilis ATCC11774.

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Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Halal Products Research Institute
DOI Number: https://doi.org/10.3390/molecules25153516
Publisher: Multidisciplinary Digital Publishing Institute
Keywords: β-mannanase; Bacillus subtilis; Palm kernel cake; Optimization; Response surface methodology
Depositing User: Ms. Nuraida Ibrahim
Date Deposited: 05 Oct 2021 22:41
Last Modified: 05 Oct 2021 22:41
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.3390/molecules25153516
URI: http://psasir.upm.edu.my/id/eprint/88817
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