UPM Institutional Repository

Enhancement of biomass and calcium carbonate biomineralization of Chlorella vulgaris through Plackett–Burman screening and Box–Behnken optimization approach


Citation

Chin, Zheng Wei and Arumugam, Kavithraashree and Ashari, Siti Efliza and Faizal Wong, Fadzlie Wong and Tan, Joo Shun and Ariff, Arbakariya and Mohamed, Mohd Shamzi (2020) Enhancement of biomass and calcium carbonate biomineralization of Chlorella vulgaris through Plackett–Burman screening and Box–Behnken optimization approach. Molecules, 25 (15). pp. 1-19. ISSN 1420-3049

Abstract

The biosynthesis of calcium carbonate (CaCO3) minerals through a metabolic process known as microbially induced calcium carbonate precipitation (MICP) between diverse microorganisms, and organic/inorganic compounds within their immediate microenvironment, gives rise to a cementitious biomaterial that may emerge as a promissory alternative to conventional cement. Among photosynthetic microalgae, Chlorella vulgaris has been identified as one of the species capable of undergoing such activity in nature. In this study, response surface technique was employed to ascertain the optimum condition for the enhancement of biomass and CaCO3 precipitation of C. vulgaris when cultured in Blue-Green (BG)-11 aquaculture medium. Preliminary screening via Plackett–Burman Design showed that sodium nitrate (NaNO3), sodium acetate, and urea have a significant effect on both target responses (p < 0.05). Further refinement was conducted using Box–Behnken Design based on these three factors. The highest production of 1.517 g/L C. vulgaris biomass and 1.143 g/L of CaCO3 precipitates was achieved with a final recipe comprising of 8.74 mM of NaNO3, 61.40 mM of sodium acetate and 0.143 g/L of urea, respectively. Moreover, polymorphism analyses on the collected minerals through morphological examination via scanning electron microscopy and crystallographic elucidation by X-ray diffraction indicated to predominantly calcite crystalline structure.


Download File

[img] Text (Abstract)
PLAC.pdf

Download (119kB)
Official URL or Download Paper: https://www.mdpi.com/1420-3049/25/15/3416

Additional Metadata

Item Type: Article
Divisions: Faculty of Science
DOI Number: https://doi.org/10.3390/molecules25153416
Publisher: Multidisciplinary Digital Publishing Institute
Keywords: Chlorella vulgaris; Box–Behnken design; Plackett–Burman design; Biomass; Calcium carbonate; Biomineralization; Response surface methodology
Depositing User: Ms. Nuraida Ibrahim
Date Deposited: 06 Oct 2021 03:11
Last Modified: 06 Oct 2021 03:11
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.3390/molecules25153416
URI: http://psasir.upm.edu.my/id/eprint/88806
Statistic Details: View Download Statistic

Actions (login required)

View Item View Item