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Efficient feeding strategy to enhance the start-up of anaerobic ammonium oxidation process in an anaerobic up-flow biofilm column reactor


Citation

Ibrahim, Mumtazah and Yusof, Norjan and Abdullah, Hanisom and Mohd Yusoff, Mohd Zulkhairi and Hassan, Mohd Ali (2020) Efficient feeding strategy to enhance the start-up of anaerobic ammonium oxidation process in an anaerobic up-flow biofilm column reactor. Desalination and Water Treatment, 183. 253 - 267. ISSN 1944-3994; ESSN: 1944-3986

Abstract

Anaerobic ammonium oxidation (anammox) performed by bacteria that belong to the Planctomycetes phylum could potentially be applied to treat wastewater with high ammonium content. However, anammox application is hindered by the slow growth of bacteria and nitrite and free ammonia inhibition. Thus, this study aims to enrich anammox culture in a 1.0 L anaerobic up-flow biofilm column reactors equipped with a non-woven fabric material, under three different feeding modes: (i) batch, (ii) fed-batch and (iii) continuous, to enhance the start-up of anammox process. A 16S rDNA gene analysis targeting planctomycetes-anammox bacteria was performed on various sludge sources to screen the appropriate inoculum of anammox microorganisms prior to the enrichment study. A reactor with a continuous mode of feeding showed the most effective consumption of N–NH4+ and N– NO2–, with the highest nitrogen removal rate of 0.28 kg N m–3 d–1 at 0.6 d hydraulic retention time and 27°C, compared to the fed-batch and batch of 0.14 and 0.004 kg N m–3 d–1, respectively. The enriched anammox culture showed as close to the ‘Candidatus Kuenenia stuttgartiensis’ genera based on 16S rDNA analysis. Fluorescence in-situ hybridization analysis targeting the 16S rDNA gene of anammox bacteria further confirmed the existence of an anammox population in the enriched culture.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Institute of Tropical Forestry and Forest Products
DOI Number: https://doi.org/10.5004/dwt.2020.25238
Publisher: Balaban Publishers – Desalination Publications
Keywords: Anaerobic up-flow; Anammox; FISH; Deammonification; Nitric oxide removal
Depositing User: Ms. Nuraida Ibrahim
Date Deposited: 06 Oct 2021 08:49
Last Modified: 06 Oct 2021 08:49
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.5004/dwt.2020.25238
URI: http://psasir.upm.edu.my/id/eprint/88755
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