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Effect of lignosulphonates on Vanilla planifolia shoot multiplication, regeneration and metabolism


Wan Abdullah, Wan Muhamad Asrul Nizam and Low, Lee Yoon and Mohd Ramli Alzaki, Siti Bahijah Mumaiyizah and Qian, Yee Chai and Loh, Jiun Yan and Abdullah, Janna Ong and Lai, Kok Song (2020) Effect of lignosulphonates on Vanilla planifolia shoot multiplication, regeneration and metabolism. Acta Physiologiae Plantarum, 42. art. no. 107. pp. 1-8. ISSN 0137-5881; ESSN: 1861-1664


Vanilla planifolia (V. planifolia) is a valuable orchidaceous plant, commonly grown for its pods that are used to produce the flavouring vanilla extract. Here, we evaluated the effect of calcium lignosulphonate (Ca-LIGN) and sodium lignosulphonate (Na-LIGN) on multiplication and regeneration of V. planifolia shoot tip culture. In 150 mg L−1 Ca-LIGN medium, the most number of shoots per explant (5.78 ± 0.63) was successfully obtained. Besides, Ca-LIGN also enhanced the shoot bud and primordial formation rate, as seen under scanning electron microscopy. In contrast, medium containing 150 mg L−1 Na-LIGN recorded the highest average of shoot length (4.72 ± 0.30 cm). Meanwhile, the best growth of root length (1.8 ± 0.32 cm) and root induction (96.67 ± 5.16%) were recorded on the explants treated with 150 mg L−1 Na-LIGN rooting medium. All rooted plantlets successfully acclimatized in the greenhouse (100.00% survival rate). Further biochemical analysis revealed that Ca-LIGN increased the total protein, chlorophyll, sugar, flavonoid and phenolic contents of V. planifolia. Notably, expression of both ribulose-1,5-bisphosphate carboxylase (Rubisco) and phosphoenolpyruvate carboxylase (PEPC) genes were also elevated under the treatment of Ca-LIGN, implying a positive role in the photosynthetic process. Taken together, LIGN being an environmental friendly product could be used to enhance the growth and micropropagation of V. planifolia.

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Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
DOI Number: https://doi.org/10.1007/s11738-020-03099-9
Publisher: Springer
Keywords: Calcium lignosulphonate; Photosynthesis; Plant growth; Sodium lignosulphonate; Vanilla planifolia
Depositing User: Ms. Nuraida Ibrahim
Date Deposited: 14 Dec 2021 23:47
Last Modified: 14 Dec 2021 23:47
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1007/s11738-020-03099-9
URI: http://psasir.upm.edu.my/id/eprint/88616
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