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Detection of Candidatus phytoplasma asteris’ (16srI) associated with bitter gourd leaf and floral malformations in Malaysia


Citation

Darshanie, Tennakon Mudiyanselage Nadika and Ahmad, Khairulmazmi and Kong, Lih Ling and Vadamalai, Ganesan (2020) Detection of Candidatus phytoplasma asteris’ (16srI) associated with bitter gourd leaf and floral malformations in Malaysia. International Journal of Sciences: Basic and Applied Research, 52 (1). 154 - 163. ISSN 2307-4531

Abstract

Bitter gourd vines (Momordica charantia) exhibiting symptoms of leaf and floral malformations including reduced leaf and flower size and shortened internodes were observed in farmer’s fields in Selangor, Malaysia. The causal agent was detected by nested and semi nested Polymerase Chain Reaction (PCR) using phytoplasma universal primers based on 16SrRNA and SecA gene sequences. Sequence analysis of 1.2 kb and 480 bp amplicons of the 16SrRNA and SecA gene respectively confirmed the presence of phytoplasma DNA associated with Candidatus phytoplasma asteris (Group16SrI) in the symptomatic bitter gourd samples. Phylogenetic analysis of the 16SrDNA and SecA sequences placed the bitter gourd phytoplasma in the 16SrI phytoplasma group. This is the first report of phytoplasma infection in bitter gourd in Malaysia.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Agriculture
Institute of Plantation Studies
Publisher: Global Society of Scientific Research and Researchers
Keywords: Phytoplasma; Bitter gourd; Molecular identification; Malaysia
Depositing User: Ms. Nuraida Ibrahim
Date Deposited: 28 Dec 2021 08:56
Last Modified: 28 Dec 2021 08:56
URI: http://psasir.upm.edu.my/id/eprint/88381
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