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Cryopreservation of oil palm (Elaeis guineensis Jacq.) polyembryoids via encapsulation–desiccation


Palanyandy, Sharrmila Rengeswari and Gantait, Saikat and Subramaniam, Sreeramanan and Sinniah, Uma Rani (2020) Cryopreservation of oil palm (Elaeis guineensis Jacq.) polyembryoids via encapsulation–desiccation. 3 Biotech, 10. art. no. 9. pp. 1-9. ISSN 2190-5738


The current report assesses the efficiency of encapsulation–desiccation protocol to cryopreserve oil palm (Elaeis guineensis Jacq.) polyembryoids. Specifically identified polyembryoids, comprising of haustorium and torpedo-shaped structures, were encapsulated [comprising 3% (w/v) sodium alginate and 100 mM CaCl2]. Calcium alginate-encapsulated and sucrose-precultured polyembryoids were subjected to different spans of desiccation in a laminar air-flow cabinet, followed by freezing in liquid nitrogen. The effect of sucrose preculture (with gradual exposure to 0.3, 0.5, 0.75 and 1 M for 7 days) and dehydration periods (0–10 h) under sterile air-flow on post-freezing survival and regrowth of encapsulated polyembryoids were studied. Cryopreserved and thawed polyembryoids (initially precultured in sucrose, followed by 9 h air-desiccated to 23.3% moisture content) displayed the highest survival percentage (73.3%) and regeneration (of shoot, root and secondary somatic embryo) on Murashige and Skoog regrowth medium containing sucrose (0.3–1 M) and 0.2 mg/l 2,4-dichlorophenoxy acetic acid. In addition, ultrastructural study using scanning electron microscopy exhibited successful revival of cryopreserved polyembryoids, owing to retention of cellular membrane stability through optimized and protected (encapsulated) desiccation. The present study thus substantiates the potential of this encapsulation–desiccation procedure in cryopreservation of oil palm polyembryoids for long-term conservation programs.

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Additional Metadata

Item Type: Article
Divisions: Faculty of Agriculture
DOI Number: https://doi.org/10.1007/s13205-019-1997-9
Publisher: Springer
Keywords: Developmental stage; Dehydration; Preculture; Regrowth; Scanning electron microscopy
Depositing User: Ms. Nuraida Ibrahim
Date Deposited: 10 Mar 2022 01:40
Last Modified: 24 Nov 2022 02:10
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1007/s13205-019-1997-9
URI: http://psasir.upm.edu.my/id/eprint/88312
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