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Persistence of malachite green and leucomalachite green in red tilapia (Oreochromis hybrid) exposedtodifferenttreatmentregimens


Kwan, Penz Penz and Banerjee, Sanjoy and Shariff, Mohamed and Md. Yusoff, Fatimah (2020) Persistence of malachite green and leucomalachite green in red tilapia (Oreochromis hybrid) exposedtodifferenttreatmentregimens. Food Control, 108. art. no. 106866. pp. 1-6. ISSN 0956-7135


Abstract Malachite green (MG) is not permitted by the EU and FDA to be used in animals intended for human consumption due to its potential carcinogenesis. However, recent findings have shown residues of MG in fish sold to consumers. Therefore, the presence of MG residues is of concern. In this study, MG and its primary metabolite leucomalachite green (LMG) in red tilapia (Oreochromis hybrid) muscle tissue was quantified by a simplified method for sample preparation using liquid chromatography-tandem mass spectrometry. The decision limit and detection capability for MG was 0.05 μg/kg and 0.09 μg/kg and for LMG was 0.05 μg/kg and 0.08 μg/kg, respectively. In addition, accuracy, precision, recovery, and matrix effects were also performed. To evaluate the persistence of MG and LMG, fish were exposed to dip (A) (66.7 mg/L; 30 s), dips (B) (66.7 mg/L; 30 s repeated for 5 days), short-term bath (C) (6.7 mg/L; 15 min), short-term bath (D) (6.7 mg/L; 15 min repeated for 5 days) and long-term bath (E) (0.5 mg/L; 6 days) treatments using MG. The MG and LMG residues were quantified at the following intervals; immediately after treatment, 1st, 2nd, 3rd, 4th, 10th, 20th and 30th day post-exposure. Long-term bath (E) showed the highest accumulation of total MG and LMG immediately after treatment (441.38 μg/kg) and at day 30 (13.30 μg/kg). Whereas, all other treatments (day 30) were below 0.26 μg/kg.

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Additional Metadata

Item Type: Article
Divisions: Faculty of Agriculture
Faculty of Veterinary Medicine
Institute of Bioscience
DOI Number: https://doi.org/10.1016/j.foodcont.2019.106866
Publisher: Elsevier
Keywords: LC-MS/MS; Limit of detection; Limit of quantification; Malachite green; Residues; Tilapia (Oreochromis hybrid)
Depositing User: Mohamad Jefri Mohamed Fauzi
Date Deposited: 13 Jan 2023 09:01
Last Modified: 13 Jan 2023 09:01
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1016/j.foodcont.2019.106866
URI: http://psasir.upm.edu.my/id/eprint/87128
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