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Optimization of protective agents for the freeze-drying of Paenibacillus polymyxa Kp10 as a potential biofungicide


Nasran, Hayatun Syamila and Mohd. Yusof, Hidayat and Halim, Murni and Abdul Rahman, Nor’Aini (2020) Optimization of protective agents for the freeze-drying of Paenibacillus polymyxa Kp10 as a potential biofungicide. Molecules, 25 (11). pp. 1-11. ISSN 1420-3049


Anthracnose is a fungal disease causing major losses in crop production. Chemical fungicides widely used in crop plantations to combat fungal infections can be a threat to the environment and humans in the long term. Recently, biofungicides have gained much interest as an alternative to chemical fungicides due to their environmentally friendly nature. Biofungicide products in powder form can be formulated using the freeze-drying technique to provide convenient storage. Protective agent formulation is needed in maintaining the optimal viable cells of biofungicide products. In this study, 8.10 log colony-forming unit (CFU)/mL was the highest cell viability of Paenibacillus polymyxa Kp10 at 22 h during incubation. The effects of several selected protective agents on the viability of P. polymyxa Kp10 after freeze-drying were studied. Response surface methodology (RSM) was used for optimizing formulation for the protective agents. The combination of lactose (10% w/v), skim milk (20% w/v), and sucrose (27.5% w/v) was found to be suitable for preserving P. polymyxa Kp10 during freeze-drying. Further, P. polymyxa Kp10 demonstrated the ability to inhibit fungal pathogens, Colletotrichum truncatum and C. gloeosporioides, at 60.18% and 66.52% of inhibition of radial growth, respectively.

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Official URL or Download Paper: https://www.mdpi.com/1420-3049/25/11/2618

Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
DOI Number: https://doi.org/10.3390/molecules25112618
Publisher: Multidisciplinary Digital Publishing Institute
Keywords: Antimicrobial; Antifungal; Biofungicide; Optimization; Paenibacillus polymyxa; RSM
Depositing User: Mohamad Jefri Mohamed Fauzi
Date Deposited: 10 Jan 2022 08:17
Last Modified: 10 Jan 2022 08:17
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.3390/molecules25112618
URI: http://psasir.upm.edu.my/id/eprint/87011
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