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Newly isolated alkane hydroxylase and lipase producing Geobacillus and Anoxybacillus species involved in crude oil degradation


Citation

Yusoff, Durratul Fatini and Raja Abdul Rahman, Raja Noor Zaliha and Masomian, Malihe and Mohamad Ali, Mohd Shukuri and Leow, Thean Chor (2020) Newly isolated alkane hydroxylase and lipase producing Geobacillus and Anoxybacillus species involved in crude oil degradation. Catalysts, 10 (8). art. no. 851. pp. 1-15. ISSN 2073-4344

Abstract

Isolation and studies of novel, crude oil biodegrading thermophilic strains may provide a wider knowledge in understanding their role in petroleum degradation. In this study, the screening of ten new thermophilic strains revealed that all strains were alkane hydroxylase producers and seven of them produced lipase concurrently. Three best strains were characterized and identified through 16S rRNA sequence analysis as Geobacillus sp. D4, Geobacillus sp. D7, and Anoxybacillus geothermalis D9 with GenBank accession numbers MK615934.1, MK615935.1, and MK615936.1, respectively. Gas chromatography (GC) analysis showed that all three strains were able to breakdown various compounds in crude oil such as alkanes, toxic poly-aromatic hydrocarbons (PAHs), organosulfur, carboxylic acids, alkene, resins, organosilicon, alcohol, organochlorine, and ester. For the first time, alkane hydroxylase and lipase activity as well as crude oil degradation by A. geothermalis species were reported. Geobacillus sp. D7 is the best alkane degrader followed by A. geothermalis D9 and Geobacillus sp. D4 with 17.3%, 13.1%, and 12.1% biodegradation efficiency (BE%), respectively. The potential of thermophiles isolated can be explored further for bioremediation of sites polluted by petroleum and oil spills.


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Official URL or Download Paper: https://www.mdpi.com/2073-4344/10/8/851

Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
DOI Number: https://doi.org/10.3390/catal10080851
Publisher: Multidisciplinary Digital Publishing Institute
Keywords: Alkane hydroxylase; Crude oil; Biodegradation; PAHs; Thermophiles; Lipase
Depositing User: Mohamad Jefri Mohamed Fauzi
Date Deposited: 03 Jan 2022 03:03
Last Modified: 03 Jan 2022 03:03
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi= 10.3390/catal10080851
URI: http://psasir.upm.edu.my/id/eprint/86899
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