UPM Institutional Repository

Main structural targets for engineering lipase substrate specificity


Albayati, Samah Hashim and Masomian, Malihe and Ishak, Siti Nor Hasmah and Mohamad Ali, Mohd Shukuri and Leow, Adam Thean Chor and Mohd Shariff, Fairolniza and Muhd Noor, Noor Dina and Raja Abdul Rahman, Raja Noor Zaliha (2020) Main structural targets for engineering lipase substrate specificity. Catalysts, 10 (7). pp. 1-43. ISSN 2073-4344


Microbial lipases represent one of the most important groups of biotechnological biocatalysts. However, the high-level production of lipases requires an understanding of the molecular mechanisms of gene expression, folding, and secretion processes. Stable, selective, and productive lipase is essential for modern chemical industries, as most lipases cannot work in different process conditions. However, the screening and isolation of a new lipase with desired and specific properties would be time consuming, and costly, so researchers typically modify an available lipase with a certain potential for minimizing cost. Improving enzyme properties is associated with altering the enzymatic structure by changing one or several amino acids in the protein sequence. This review detailed the main sources, classification, structural properties, and mutagenic approaches, such as rational design (site direct mutagenesis, iterative saturation mutagenesis) and direct evolution (error prone PCR, DNA shuffling), for achieving modification goals. Here, both techniques were reviewed, with different results for lipase engineering, with a particular focus on improving or changing lipase specificity. Changing the amino acid sequences of the binding pocket or lid region of the lipase led to remarkable enzyme substrate specificity and enantioselectivity improvement. Site-directed mutagenesis is one of the appropriate methods to alter the enzyme sequence, as compared to random mutagenesis, such as error-prone PCR. This contribution has summarized and evaluated several experimental studies on modifying the substrate specificity of lipases.

Download File

[img] Text
Main structural targets .pdf

Download (8kB)

Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
DOI Number: https://doi.org/10.3390/catal10070747
Publisher: Multidisciplinary Digital Publishing Institute
Keywords: Lipase; Specificity; Binding pocket; Lid; Oxyanion hole; Protein engineering; Chemoselectivity; Regioselectivity; Stereoselectivity
Depositing User: Mohamad Jefri Mohamed Fauzi
Date Deposited: 13 Oct 2021 05:23
Last Modified: 13 Oct 2021 05:23
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.3390/catal10070747
URI: http://psasir.upm.edu.my/id/eprint/86618
Statistic Details: View Download Statistic

Actions (login required)

View Item View Item