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Lipid signaling pathways genes expression level in HEPG2 cells supplemented with exogenous lipid and edible bird nest (EBN) extract


Citation

Tawai, Darreen (2018) Lipid signaling pathways genes expression level in HEPG2 cells supplemented with exogenous lipid and edible bird nest (EBN) extract. [Project Paper Report]

Abstract

“Caviar of the East” or better known as Edible-Bird Nest (EBN) is a highly valuable product composed from solidified salivary secretion of a few insectivorous swiftlet species. Swiftlets falls under the family Apodidae and the two main genera with the highest commercial values are Aerodramus (echolating swiftlets) and Collocalia (non-echolating swiftlets). The objective of this study is to determine the qualitative genes expression associated in lipid signalling pathways such as Acetyl-CoA acetyltransferase 2 (ACAT2), Diacylglycerol O-acyltransferase 2 (DGAT2), Low density lipoprotein receptor (LDLR), Sterol regulatory element binding transcription factor 2 (SREBP2), 3-hydroxy-3-methylglutaryl-CoA reductase (HMGCR), Proprotein Convertase Subtilisin/kexin type 9 (PCSK9) in HepG2 mammalian cell with or without supplementation of EBN extract and exogenous lipid. Raw EBN were manually cleaned, dried in 60°C oven and left overnight. In this project, EBN supernatant were added with acetone using 1:2 ratio and kept in -80°C for an hour. Hep G2 cells were cultured in six wells plates and each wells were treated according to Base Control, Positive Control, Negative Control, Treatment 1, Treatment 2 and Treatment 3. In this study, there are significant changes in term of genes expression with supplementation of EBN extract especially in ACAT2 and LDLR genes.


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Additional Metadata

Item Type: Project Paper Report
Call Number: FPV 2018 23
Chairman Supervisor: Dr. Mohd Mokrish Md. Ajat
Divisions: Faculty of Veterinary Medicine
Keywords: Edible bird nest; Aerodramus spp.; Lipid metabolism; Genes expression; Qualitative PCR
Depositing User: Mas Norain Hashim
Date Deposited: 06 Oct 2020 02:02
Last Modified: 06 Oct 2020 02:02
URI: http://psasir.upm.edu.my/id/eprint/83617
Statistic Details: View Download Statistic

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