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Vitamin D and Stimulates the PI3K-AKT signaling pathway in insulin resistant SK-N-SH neuronal cells


Zaulkffali, Amirah Salwani and Md Razip, Nurliyana Najwa and Syed Alwi, Sharifah Sakinah and Abd Jalil, Afifah and Abd Mutalib, Mohd Sokhini and Gopalsamy, Banulata and Chang, Sui Kiat and Zainal, Zaida and Ibrahim, Nafissa Nadia and Zakaria, Zainul Amiruddin and Khaza'ai, Huzwah (2019) Vitamin D and Stimulates the PI3K-AKT signaling pathway in insulin resistant SK-N-SH neuronal cells. Nutrients, 11 (10). pp. 1-20. ISSN 2072-6643


This study investigated the effects of vitamins D and E on an insulin-resistant model and hypothesized that this treatment would reverse the effects of Alzheimer’s disease (AD) and improves insulin signalling. An insulin-resistant model was induced in SK-N-SH neuronal cells with a treatment of 250 nM insulin and re-challenged with 100 nM at two different incubation time (16 h and 24 h). The effects of vitamin D (10 and 20 ng/mL), vitamin E in the form of tocotrienol-rich fraction (TRF) (200 ng/mL) and the combination of vitamins D and E on insulin signalling markers (IR, PI3K, GLUT3, GLUT4, and p-AKT), glucose uptake and AD markers (GSK3β and TAU) were determined using quantitative real-time polymerase chain reaction (qRT-PCR) and enzyme-linked immunosorbent assay (ELISA). The results demonstrated an improvement of the insulin signalling pathway upon treatment with vitamin D alone, with significant increases in IR, PI3K, GLUT3, GLUT4 expression levels, as well as AKT phosphorylation and glucose uptake, while GSK3β and TAU expression levels was decreased significantly. On the contrary, vitamin E alone, increased p-AKT, reduced the ROS as well as GSK3β and TAU but had no effect on the insulin signalling expression levels. The combination of vitamins D and E only showed significant increase in GLUT4, p-AKT, reduced ROS as well as GSK3β and TAU. Thus, the universal role of vitamin D, E alone and in combinations could be the potential nutritional agents in restoring the sensitivity of neuronal cells towards insulin and delaying the pathophysiological progression of AD.

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Official URL or Download Paper: https://www.mdpi.com/2072-6643/11/10/2525

Additional Metadata

Item Type: Article
Divisions: Faculty of Medicine and Health Science
DOI Number: https://doi.org/10.3390/nu11102525
Publisher: MDPI
Keywords: Insulin resistance; Vitamin D; Vitamin E; SK-N-SH neuronal cells; Glucose uptake
Depositing User: Mr. Sazali Mohamad
Date Deposited: 02 Jun 2021 09:13
Last Modified: 02 Jun 2021 09:13
Altmetrics: http://www.altmetrics.com/details.php?domain=pasir.upm.edu.my&doi=10.3390/nu11102525
URI: http://psasir.upm.edu.my/id/eprint/82729
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