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The design of a thermoresponsive surface for the continuous culture of human pluripotent stem cells


Sung, Tzu Cheng and Yang, Jia Sin and Yeh, Chih Chen and Liu, Ya Chu and Jiang, Yi Peng and Lu, Ming We and Ling, Qing Dong and Kumar, S. Suresh and Chang, Yung and Umezawa, Akihiro and Chen, Hao and Higuchi, Akon (2019) The design of a thermoresponsive surface for the continuous culture of human pluripotent stem cells. Biomaterials, 221. pp. 1-13. ISSN 0142-9612; ESSN: 1878-5905


Commonly, stem cell culture is based on batch-type culture, which is laborious and expensive. We continuously cultured human pluripotent stem cells (hPSCs) on thermoresponsive dish surfaces, where hPSCs were partially detached on the same thermoresponsive dish by decreasing the temperature of the thermoresponsive dish to be below the lower critical solution temperature for only 30 min. Then, the remaining cells were continuously cultured in fresh culture medium, and the detached stem cells were harvested in the exchanged culture medium. hPSCs were continuously cultured for ten cycles on the thermoresponsive dish surface, which was prepared by coating the surface with poly(N-isopropylacrylamide-co-styrene) and oligovitronectin-grafted poly(acrylic acid-co-styrene) or recombinant vitronectin for hPSC binding sites to maintain hPSC pluripotency. After ten cycles of continuous culture on the thermoresponsive dish surface, the detached cells expressed pluripotency proteins and had the ability to differentiate into cells derived from the three germ layers in vitro and in vivo. Furthermore, the detached cells differentiated into specific cell lineages, such as cardiomyocytes, with high efficiency.

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Additional Metadata

Item Type: Article
Divisions: Faculty of Medicine and Health Science
DOI Number: https://doi.org/10.1016/j.biomaterials.2019.119411
Publisher: Elsevier
Keywords: Biomaterial; Cardiomyocyte; Human embryonic stem cell; Thermoresponsive surface; Xeno-free culture
Depositing User: Mr. Sazali Mohamad
Date Deposited: 28 Aug 2021 00:00
Last Modified: 28 Aug 2021 00:01
Altmetrics: http://www.altmetrics.com/details.php?domain=pasir.upm.edu.my&doi=10.1016/j.biomaterials.2019.119411
URI: http://psasir.upm.edu.my/id/eprint/82380
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