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Spray-dried immobilized lipase from Geobacillus sp. strain ARM in sago


Mohtar, Nur Syazwani and Abdul Rahman, Mohd Basyaruddin and Mustafa, Shuhaimi and Mohamad Ali, Mohd Shukuri and Raja Abd Rahman, Raja Noor Zaliha (2019) Spray-dried immobilized lipase from Geobacillus sp. strain ARM in sago. PeerJ, 31 (7). art. no. e6880. ISSN 2167-8359


Sago starch is traditionally used as food especially in Southeast Asia. Generally, sago is safe for consumption, biodegradable, easily available and inexpensive. Therefore, this research was done to expand the potential of sago by using it as a support for enzyme immobilization. In this study, ARM lipase, which was isolated from Geobacillus sp. strain ARM, was overexpressed in Escherichia coli system and then purified using affinity chromatography. The specific activity of the pure enzyme was 650 U/mg, increased 7 folds from the cell lysate. The purified enzyme was immobilized in gelatinized sago and spray-dried by entrapment technique in order to enhance the enzyme operational stability for handling at high temperature and also for storage. The morphology of the gelatinized sago and immobilized enzyme was studied by scanning electron microscopy. The results showed that the spray-dried gelatinized sago was shrunken and became irregular in structure as compared to untreated sago powder. The surface areas and porosities of spray-dried gelatinized sago with and without the enzyme were analyzed using BET and BJH method and have shown an increase in surface area and decrease in pore size. The immobilized ARM lipase showed good performance at 60-80 °C, with a half-life of 4 h and in a pH range 6-9. The immobilized enzyme could be stored at 10 °C with the half-life for 9 months. Collectively, the spray-dried immobilized lipase shows promising capability for industrial uses, especially in food processing.

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Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Faculty of Science
Halal Products Research Institute
DOI Number: https://doi.org/10.7717/peerj.6880
Publisher: PeerJ
Keywords: Entrapment; Enzyme immobilization; Geobacillus sp.; Lipase; Sago; Spray-dry
Depositing User: Mr. Sazali Mohamad
Date Deposited: 16 Dec 2020 06:30
Last Modified: 16 Dec 2020 06:30
Altmetrics: http://www.altmetrics.com/details.php?domain=pasir.upm.edu.my&doi=10.7717/peerj.6880
URI: http://psasir.upm.edu.my/id/eprint/82228
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