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Silver nanoparticles Clinacanthus nutans leaves extract induced apoptosis towards oral squamous cell carcinoma cell lines


Yakop, Fahmi and Abd Ghafar, Siti Aisyah and Yong, Yoke Keong and Saiful Yazan, Latifah and Mohamad Hanafiah, Rohazila and Lim, Vuanghao and Eshak, Zolkapli (2019) Silver nanoparticles Clinacanthus nutans leaves extract induced apoptosis towards oral squamous cell carcinoma cell lines. Artificial Cells Nanomedicine and Biotechnology, 46 (suppl. 2). pp. 131-139. ISSN 2169-1401; ESSN: 2169-141X


Purpose:The purpose of this study was to investigate apoptotic activity of silver nanoparticle Clinacanthus nutans(AgNps-CN) towards HSC-4 cell lines (oral squamous cell carcinoma cell lines).Methods:Methods involved were MTT assay (cytotoxic activity), morphological cells analysis, flow cytometry and cell cycle analysis and western blot.Results:MTT assay revealed IC50concentration was 1.61mg/mL, 3T3-L1 cell lines were used to deter-mine whether AgNps-CN is cytotoxic to normal cells. At the highest concentration (3mg/mL), no cyto-toxic activity has been observed. Flow cytometry assay revealed AgNps-CN caused apoptosis effects towards HSC-4 cell lines with significant changes were observed at G1 phase when compared with untreated cells. Morphological cells analysis revealed that most of the cells exhibit apoptosis characteristics rather than necrosis. Protein study revealed that ratio of Bax/Bcl-2 increased mainly due to down-regulation of Bcl-2 expression.Conclusion:AgNps-CN have shown potential in inhibiting HSC-4 cell lines. IC50was low compared to few studies involving biosynthesized of silver nanoparticles. Apoptosis effects were shown towardsHSC-4 cell lines by the increased in Bax/Bcl-2 protein ratio. Further study such as PCR or in vivo studies are required.

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Additional Metadata

Item Type: Article
Divisions: Faculty of Medicine and Health Science
DOI Number: https://doi.org/10.1080/21691401.2018.1452750
Publisher: Taylor & Francis Open Access
Keywords: Silver nanoparticle; Clinacanthus nutans; Oralcancer; Flow cytometry
Depositing User: Mr. Sazali Mohamad
Date Deposited: 08 Sep 2021 11:06
Last Modified: 08 Sep 2021 11:06
Altmetrics: http://www.altmetrics.com/details.php?domain=pasir.upm.edu.my&doi=10.1080/21691401.2018.1452750
URI: http://psasir.upm.edu.my/id/eprint/82123
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