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Proteome of rice roots treated with exogenous proline


Teh, Chui Yao and Ling, Chai and Shaharuddin, Noor Azmi and Kok, Song Lai and Mahmood, Maziah (2019) Proteome of rice roots treated with exogenous proline. 3 Biotech, 9. art. no. 101. pp. 1-8. ISSN 2190-572X; ESSN: 2190-5738


Proteomic analysis was conducted to identify the rice root proteins induced by exogenous proline and their involvement in root growth. Proteins were extracted from the root tissues grown under two conditions, T1 (control) and T2 (10 mM proline), and profiled by two-dimensional polyacrylamide gel electrophoresis. Seventeen of 30 differentially expressed proteins were identified by mass spectrometry. Proline-treated rice roots showed up-regulation and down-regulation of nine and eight proteins, respectively, when compared to those in the control. Among the differentially expressed proteins, the down-regulation of glutathione reductase and peroxidase could be involved in the regulation of cellular hydrogen peroxide and reactive oxygen species levels that modulate the root cell wall structure. Differentially expressed proteins identified as pathogenesis-related proteins might be related to stress adaptive mechanisms in response to exogenous proline treatment. In addition, differentially expressed protein identified as the fructose-bisphosphate aldolases and cytochrome c oxidase might be associated with energy metabolism, which is needed during root developmental process. This is the first attempt to study the changes in rice root proteome treated with proline. The acquired information could open new avenues for further functional studies on the involvement of proline in modulating root development and its relation to stress adaptation of plants.

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Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Institute of Plantation Studies
DOI Number: https://doi.org/10.1007/s13205-019-1615-x
Publisher: Springer Verlag
Keywords: Proline; Protein; Rice; Root
Depositing User: Mr. Sazali Mohamad
Date Deposited: 20 Aug 2021 23:03
Last Modified: 20 Aug 2021 23:03
Altmetrics: http://www.altmetrics.com/details.php?domain=pasir.upm.edu.my&doi=10.1007/s13205-019-1615-x
URI: http://psasir.upm.edu.my/id/eprint/81876
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