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Production of ligninolytic enzymes by Coptotermes curvignathus gut bacteria


Citation

Ayeronfe, Fadilah and Kassim, Angzzas and Hung, Patricia and Ishak, Nadiah and Sharfina Syarifah and Aripin, Ashuvila (2019) Production of ligninolytic enzymes by Coptotermes curvignathus gut bacteria. Environmental and Climate Technologies, 23 (1). pp. 111-121. ISSN 16915208

Abstract

Maximum utilization of lignocellulosic biomass is contingent upon degrading the recalcitrant lignin polymer. Conventional methods employed in delignification require high inputs of energy and chemicals, resulting in the release of highly toxic effluents. The ability of gut flora of Coptotermes curvignathus in lignin degradation was investigated in this study. Production of ligninolytic enzymes was done in an aerated submerged fermentation system with kraft lignin as sole carbon source. The degradation experiment was carried out for 7 days at 30 °C, pH 7. Three potential lignin degraders identified as Bacillus sp., Lysinibacillus sp. and Acinetobacter sp. were successfully isolated. The bacterial growth and secretion of extracellular ligninolytic enzymes confirmed metabolism of kraft lignin by the identified strains. Lysinibacillus sp., a novel lignin degrader showed highest manganese peroxidase (76.36 ± 15.74 U/L) and laccase activity (70.67 ± 16.82 U/L) after 7 and 6 days of incubation respectively, while maximal activity of lignin peroxidase (262.49 ± 0.92 U/L) was recorded after 7 days in culture supernatants of Bacillus sp. With respect to the activity of the secreted enzymes, the lignin degrading potential of these bacterial strains can be explored in the valorisations of lignocellulosic biomass in industrial processes such as pulping, bioethanol production, fine chemicals and materials synthesis.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Agriculture and Food Sciences
DOI Number: https://doi.org/10.2478/rtuect-2019-0008
Publisher: Walter de Gruyter GmbH
Keywords: Enzymes; Kraft lignin; Laccase; Peroxidases; Termites
Depositing User: Ms. Nida Hidayati Ghazali
Date Deposited: 27 Aug 2020 02:23
Last Modified: 27 Aug 2020 02:23
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.2478/rtuect-2019-0008
URI: http://psasir.upm.edu.my/id/eprint/81849
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