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Neuroprotective effects of glucomoringin-isothiocyanate against H₂O₂-Induced cytotoxicity in neuroblastoma (SH-SY5Y) cells


Jaafaru, Mohammed Sani and Nordin, Norshariza and Rosli, Rozita and Shaari, Khozirah and Bako, Hauwa'u Yakubu and Saad, Norazalina and Mohd Noor, Noramaliza and Abdull Razis, Ahmad Faizal (2019) Neuroprotective effects of glucomoringin-isothiocyanate against H₂O₂-Induced cytotoxicity in neuroblastoma (SH-SY5Y) cells. NeuroToxicology, 75. pp. 89-104. ISSN 1872-9711; ESSN: 0161-813X


Neurodegenerative diseases (NDDs) are pathological conditions characterised by progressive damage of neuronal cells leading to eventual loss of structure and function of the cells. Due to implication of multi-systemic complexities of signalling pathways in NDDs, the causes and preventive mechanisms are not clearly delineated. The study was designed to investigate the potential signalling pathways involved in neuroprotective activities of purely isolated glucomoringin isothiocyanate (GMG-ITC) against H₂O₂-induced-induced cytotoxicity in neuroblastoma (SH-SY5Y) cells. GMG-ITC was isolated from Moringa oleifera seeds, and confirmed with NMR and LC-MS based methods. Gene expression analysis of phase II detoxifying markers revealed significant increase in the expression of all the genes involved, due to GMG-ITC pre-treatment. GMG-ITC also caused significant decreased in the expression of NF-kB, BACE1, APP and increased the expressions of IkB and MAPT tau genes in the differentiated cells as confirmed by multiplex genetic system analysis. The effect was reflected on the expressed proteins in the differentiated cells, where GMG-ITC caused increased in expression level of Nrf2, SOD-1, NQO1, p52 and c-Rel of nuclear factor erythroid factor 2 (Nrf2) and nuclear factor kappa-B (NF-kB) pathways respectively. The findings revealed the potential of GMG-ITC to abrogate

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Official URL or Download Paper: https://pubmed.ncbi.nlm.nih.gov/31521693/

Additional Metadata

Item Type: Article
Divisions: Faculty of Medicine and Health Science
Institute of Bioscience
DOI Number: https://doi.org/10.1016/j.neuro.2019.09.008
Publisher: Elsevier
Keywords: Glucomoringin isothiocyanate; Neuroblastoma; Neurodegeneration; Neuroprotection; SH-SY5Y differentiation; Signalling pathways
Depositing User: Ms. Nida Hidayati Ghazali
Date Deposited: 30 Jan 2021 18:51
Last Modified: 30 Jan 2021 18:51
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1016/j.neuro.2019.09.008
URI: http://psasir.upm.edu.my/id/eprint/81465
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