UPM Institutional Repository

Malaysian Tualang honey inhibits hydrogen peroxide-induced endothelial hyperpermeability


Devasvaran, Kogilavanee and Tan, Jun Jie and Theng, Ng Theng and Lai, Yen Fong and Yong, Yoke Keong (2019) Malaysian Tualang honey inhibits hydrogen peroxide-induced endothelial hyperpermeability. Oxidative Medicine and Cellular Longevity, 2019. art. no. 1202676. pp. 1-10. ISSN 1942-0900; ESSN: 1942-0994


Malaysian Tualang honey (TH) is a known therapeutic honey extracted from the honeycombs of the Tualang tree (Koompassia excelsa) and has been reported for its antioxidant, anti-inflammatory, antiproliferative, and wound healing properties. However, the possible vascular protective effect of TH against oxidative stress remains unclear. In this study, the effects of TH on hydrogen peroxide- (H2O2-) elicited vascular hyperpermeability in human umbilical vein endothelial cells (HUVECs) and Balb/c mice were evaluated. Our data showed that TH concentrations ranging from 0.01% to 1.00% showed no cytotoxic effect to HUVECs. Induction with 0.5 mM H2O2 was found to increase HUVEC permeability, but the effect was significantly reversed attenuated by TH (p < 0 05), of which the permeability with the highest inhibition peaked at 0.1%. In Balb/c mice, TH (0.5 g/kg-1.5 g/kg) significantly (p < 0 05) reduced H2O2 (0.3%)-induced albumin-bound Evans blue leak, in a dose-dependent manner. Immunofluorescence staining confirmed that TH reduced actin stress fiber formation while increasing cortical actin formation and colocalization of caveolin-1 and β-catenin in HUVECs. Signaling studies showed that HUVECs pretreated with TH significantly (p < 0 05) decreased intracellular calcium release, while sustaining the level of cAMP when challenged with H2O2. These results suggested that TH could inhibit H2O2-induced vascular hyperpermeability in vitro and in vivo by suppression of adherence junction protein redistribution via calcium and cAMP, which could have a therapeutic potential for diseases related to the increase of both oxidant and vascular permeability.

Download File

[img] Text
Malaysian Tualang honey .pdf

Download (63kB)

Additional Metadata

Item Type: Article
Divisions: Faculty of Medicine and Health Science
DOI Number: https://doi.org/10.1155/2019/1202676
Publisher: Hindawi Publishing Corporation
Keywords: Malaysian Tualang honey; Tualang; Immunofluorescence; Vascular
Depositing User: Ms. Nida Hidayati Ghazali
Date Deposited: 14 Jun 2021 23:35
Last Modified: 14 Jun 2021 23:35
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1155/2019/1202676
URI: http://psasir.upm.edu.my/id/eprint/81272
Statistic Details: View Download Statistic

Actions (login required)

View Item View Item